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Consecuencias de la desregulación generalizada del gen c-myc en ratones transgénicos: neoplasias múltiples y
Cell
|May 23, 1986
Resumen
Los ratones transgénicos con un oncogén c-myc inducible desarrollaron varios tumores, pero mantuvieron un desarrollo normal. Esto sugiere que se necesitan factores adicionales para la malignidad, que definen el espectro de transformación del oncogén c-myc.
Área de la Ciencia:
- Oncología Oncología.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- El oncogén c-myc juega un papel crítico en la proliferación y diferenciación celular.
- La desregulación de c-myc está implicada en el desarrollo de numerosos tipos de cáncer.
- Comprender el papel preciso de c-myc en la tumorigénesis requiere estudiar sus efectos in vivo.
Objetivo del estudio:
- Para investigar el potencial oncogénico de un transgénero c-myc desregulado, inducible por glucocorticoides.
- Para determinar el rango de tumores que pueden surgir de la activación de c-myc en un organismo vivo.
- Para definir el espectro de transformación del oncogén c-myc e identificar los factores necesarios para la malignidad.
Principales métodos:
- Construcción de un modelo de ratón transgénico que expresa un gen de fusión LTR/c-myc del virus del tumor mamario.
- Inducción de la expresión transgénica mediante el uso de glucocorticoides.
- El monitoreo de la incidencia de tumores y la caracterización de los tipos de tumores en varios tejidos.
- Evaluar el impacto del transgénico en la proliferación y el desarrollo de las células normales.
Principales resultados:
- La expresión anormal del transgén c-myc se produjo en múltiples tejidos.
- Se observó un aumento de la incidencia de tumores en los linajes de células testiculares, mamarias, linfocíticas (células B y T) y de mastocitos.
- El transgén c-myc desregulado no interrumpió la proliferación o el desarrollo celular normal.
- Las neoplasias no se desarrollaron en todos los tejidos que expresan el transgen, lo que indica respuestas específicas del tejido.
Conclusiones:
- La c-myc activada por sí sola es insuficiente para inducir la malignidad en todos los tejidos.
- Factores celulares o ambientales adicionales son necesarios para la tumorigénesis junto con c-myc.
- Este estudio ayuda a definir el espectro de transformación del oncogén c-myc y sus requisitos para inducir el cáncer.
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