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Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

Los transistores de efecto de campo Aptamer superan las limitaciones de longitud de Debye para la detección de

Nako Nakatsuka^1,2, Kyung-Ae Yang³, John M Abendroth^1,2

¹California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA.

Science (New York, N.Y.)

|September 8, 2018

Resumen

Este estudio supera las limitaciones de longitud de Debye en los sensores de transistores de efecto de campo. Las modificaciones de Aptamer permiten la detección sensible de pequeñas moléculas, incluidos los neurotransmisores y la glucosa, en condiciones fisiológicas.

Área de la Ciencia:

Ingeniería biomédica
Nanotecnología
Química analítica

Sus antecedentes:

Los transistores de efecto de campo (FET) están limitados en la detección de analitos debido a la doble capa eléctrica (longitud de Debye).
Los métodos de biosensorización existentes luchan con la sensibilidad en condiciones fisiológicas de alta fuerza iónica.

Objetivo del estudio:

Desarrollar una nueva plataforma de detección basada en FET que supere las limitaciones de longitud de Debye.
Para lograr una detección sensible de moléculas pequeñas en condiciones fisiológicas.

Principales métodos:

Modificación de matrices FET de óxido metálico ultradelgado impresas con aptameros de desoxirribonucleótidos.
Utilizando cambios conformacionales de aptamer inducidos por el objetivo a la conductividad del canal de semiconductores de la puerta.
El uso de receptores de bucle de tallo aptaméricos específicamente aislados para el reconocimiento de objetivos.

Principales resultados:

Se ha demostrado la sensibilidad a la detección de pequeñas moléculas (serotonina, dopamina, glucosa, esfingosina-1-fosfato) en amortiguadores de alta resistencia iónica.
Superó la limitación fundamental de longitud de Debye en la detección basada en FET.
Se ha logrado la detección de analíticos cargados y electroneutrales.

Conclusiones:

La modificación adaptativa del aptamer de los FET proporciona una estrategia viable para el biosensing sensible.
Este enfoque permite una detección robusta del analito en entornos fisiológicos complejos.
La plataforma desarrollada tiene potencial para diversas aplicaciones de diagnóstico y monitoreo.

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