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Una nueva clase de proteínas de membrana ER regula la fisión endosómica asociada a ER

Melissa J Hoyer¹, Patrick J Chitwood¹, Christopher C Ebmeier¹

¹Department of Molecular, Cellular, and Developmental Biology, University of Colorado-Boulder, Boulder, CO 80309, USA.

|September 18, 2018

Resumen

Los sitios de contacto de la membrana del retículo endoplasmático (ER) son cruciales para la fisión endosómica. La proteína ER TMCC1, reclutada por la Coronina 1C, regula este proceso para la clasificación adecuada de la carga.

Área de la Ciencia:

Biología celular
Biología molecular
Tráfico de membranas

Sus antecedentes:

Los sitios de contacto de la membrana del retículo endoplasmático (ER) (MCS) son uniones celulares críticas.
Estos sitios están involucrados en la regulación de la fisión endosómica y la clasificación de la carga.
El papel preciso de la ER en estos eventos sigue siendo incompletamente entendido.

Objetivo del estudio:

Identificar las proteínas ER involucradas en la fisión endosómica en las MCS.
Para aclarar el mecanismo por el cual el ER influye en el brote de endosomas y la clasificación de carga.
Definir la relación entre las proteínas ER y los reguladores de actina en estos sitios.

Principales métodos:

Utilizó una estrategia de ligasa de biotina promiscua para identificar las proteínas ER que interactúan en los brotes endosómicos.
Estudios de agotamiento utilizando siRNA para evaluar la función de las proteínas identificadas.
Microscopia de inmunofluorescencia para visualizar la localización de proteínas y la morfología endosómica.
Investigó el papel de la coronina 1C en el reclutamiento de ER y la fisión dependiente de actina.

Principales resultados:

Se identificó TMCC1, una proteína de membrana ER, que se concentra en los MCS endosómicos ER.
El agotamiento de TMCC1 afecta la fisión endosómica asociada a ER y la clasificación de carga en el Golgi.
La coronina 1C es esencial para el reclutamiento de TMCC1 en el sitio de contacto del endosoma ER.
La fisión asociada a ER de los dominios de clasificación de carga dependientes de la actina requiere coronina 1C.

Conclusiones:

TMCC1 es una proteína clave residente en ER que regula la fisión endosómica en las MCS.
El reclutamiento de TMCC1 en los sitios de contacto del endosoma ER depende de la coronina 1C.
Esta interacción pone de relieve un mecanismo regulado para la participación de ER en la clasificación de la carga durante la fisión endosómica.

Palabras clave:

El retículo endoplasmático Fisión de los endosomas Reciclaje de endosomas Sitio de contacto con la membrana

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