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Caracterización y actividad biológica de un complejo de sulfuro de hidrógeno activado por luz roja
Joshua J Woods1,2, Jian Cao3, Alexander R Lippert3
1Robert F. Smith School for Chemical and Biomolecular Engineering , Cornell University , Ithaca , New York 14853 , United States.
Journal of the American Chemical Society
|September 20, 2018
Resumen
Los investigadores desarrollaron un nuevo agente basado en rutenio que libera sulfuro de hidrógeno (H2S) solo cuando se activa con luz roja. Esta liberación controlada protege las células cardíacas de las lesiones, ofreciendo una nueva herramienta para estudiar el potencial terapéutico de H2S.
Área de la Ciencia:
- La bioquímica
- Biología Química
- Química médica
Sus antecedentes:
- El sulfuro de hidrógeno (H2S) es un gasotransmisor crucial con potencial terapéutico para la lesión de isquemia-reperfusión.
- Los actuales predrogas de liberación H2S a menudo sufren de liberación incontrolada a través de la hidrólisis, lo que limita su aplicación terapéutica.
- La administración intracelular efectiva de H2S requiere estrategias sofisticadas de fármacos precursores.
Objetivo del estudio:
- Desarrollar un nuevo agente de liberación de H2S con control espacio-temporal.
- Investigar la activación de la liberación de H2S por irradiación de luz roja en células vivas.
- Evaluar los efectos protectores de la liberación de H2S activada por la luz contra la lesión por isquemia-reperfusión.
Principales métodos:
- Síntesis y caracterización de un compuesto liberador de H2S basado en Ru.
- Demostración de la liberación de H2S provocada por la luz roja en los cardiomioblastos H9c2 vivos.
- Evaluación de la viabilidad celular y protección contra una lesión por isquemia-reperfusión in vitro.
Principales resultados:
- Se sintetizó un compuesto a base de Ru (II) que libera selectivamente H2S al activarse con luz roja.
- Los niveles intracelulares de H2S aumentaron significativamente sólo después de la irradiación con luz roja.
- La liberación de H< sub>2S activada por luz roja confería una protección significativa a los cardiomioblastos H9c2 contra la lesión por isquemia-reperfusión.
Conclusiones:
- Los agentes de liberación de H2S activados por luz roja ofrecen un control preciso sobre la entrega del gasotransmisor.
- Esta tecnología proporciona una herramienta valiosa para investigar las funciones biológicas y las aplicaciones terapéuticas de H2S.
- El agente desarrollado demuestra potencial para la terapia dirigida en condiciones como la lesión por isquemia-reperfusión.
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