Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Second-Order Circuits01:17

Second-Order Circuits

3.6K
Integrating two fundamental energy storage elements in electrical circuits results in second-order circuits, encompassing RLC circuits and circuits with dual capacitors or inductors (RC and RL circuits). Second-order circuits are identified by second-order differential equations that link input and output signals.
Input signals typically originate from voltage or current sources, with the output often representing voltage across the capacitor and/or current through the inductor. For example, in...
3.6K
First-Order Circuits01:15

First-Order Circuits

3.8K
First-order electrical circuits, which comprise resistors and a single energy storage element - either a capacitor or an inductor, are fundamental to many electronic systems. These circuits are governed by a first-order differential equation that describes the relationship between input and output signals.
One common example of a first-order circuit is the RC (resistor-capacitor) circuit. These circuits are used in relaxation oscillators such as neon lamp oscillator circuits. When voltage is...
3.8K
The Y-to-Y Circuit01:19

The Y-to-Y Circuit

755
In a balanced four-wire wye-to-wye system, the arrangement involves wye-connected sinusoidal voltage sources and loads, connected through a neutral wire that links the neutral nodes of the source and load. The load impedance is connected across each phase of the load. The wye-connected source can be connected to the wye-connected load in four-wire and three-wire arrangements. A three-phase system is considered balanced when the load on each phase is equal, leading to uniform current flow and...
755
LC Circuits01:21

LC Circuits

3.3K
An LC circuit consists of an inductor and a capacitor, either in series or parallel. Consider a charged capacitor connected with an inductor in series. Before the switch is closed, all the energy of the circuit is stored in the electric field of the capacitor. When the switch is closed, the capacitor begins to discharge, producing a current in the circuit. The current, in turn, creates a magnetic field in the inductor. Because of the induced emf in the inductor, the current cannot change...
3.3K
Three-Phase Circuits01:22

Three-Phase Circuits

840
AC power distribution systems have three categories: single-phase, two-phase, and three-phase systems. The single-phase circuit, common in residential settings, typically employs a two-wire system connecting a single AC source to various loads. These circuits support standard household appliances operating at 120 volts (V) and 240 V, such as lamps, televisions, and microwaves. The first generators, Niagara Falls hydro plant installed in 1895, were two-phase and designed by Nikola Tesla. The...
840

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Efficient generation of epitope-targeted antibodies with Germinal.

Nature biotechnology·2026
Same author

Mapping and engineering the human cell-cell interactome.

Nature biotechnology·2026
Same author

Programmable pathway profiles reveal signaling principles of TGF-β superfamily receptors.

bioRxiv : the preprint server for biology·2026
Same author

Genome-wide chromatin recording resolves dynamic cell state changes.

bioRxiv : the preprint server for biology·2026
Same author

A single-cell screening platform accelerates functional genetics in plants.

Nature biotechnology·2026
Same author

Engineering multiple levels of specificity in an RNA viral vector.

Nature communications·2026

Video Experimental Relacionado

Updated: Feb 5, 2026

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.6K

Los circuitos de proteínas programables en las células vivas

Xiaojing J Gao1, Lucy S Chong1, Matthew S Kim1

  • 1Howard Hughes Medical Institute, Division of Biology and Biological Engineering, Broad Center, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.

Science (New York, N.Y.)
|September 22, 2018
PubMed
Resumen
Este resumen es generado por máquina.

Las proteasas virales diseñadas forman un sistema modular para la biología sintética, lo que permite funciones celulares complejas. Esta plataforma de ingeniería de circuitos de proteínas ofrece aplicaciones biotecnológicas escalables sin integración genómica.

Más Videos Relacionados

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
08:59

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Published on: February 12, 2019

11.9K
4D Imaging of Protein Aggregation in Live Cells
08:59

4D Imaging of Protein Aggregation in Live Cells

Published on: April 5, 2013

17.8K

Videos de Experimentos Relacionados

Last Updated: Feb 5, 2026

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.6K
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
08:59

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Published on: February 12, 2019

11.9K
4D Imaging of Protein Aggregation in Live Cells
08:59

4D Imaging of Protein Aggregation in Live Cells

Published on: April 5, 2013

17.8K

Área de la Ciencia:

  • Biología sintética
  • Ingeniería molecular
  • Ingeniería de células de mamíferos

Sus antecedentes:

  • La ingeniería de comportamientos celulares requiere circuitos sintéticos sofisticados.
  • Los sistemas de regulación de proteínas compuestas son cruciales para el diseño racional de circuitos.
  • Las proteasas virales ofrecen potencial como componentes de proteínas modulares.

Objetivo del estudio:

  • Desarrollar un sistema de regulación proteico-proteico para la ingeniería de las funciones celulares.
  • Para demostrar la utilidad de las proteasas virales diseñadas como componentes de circuitos modulares.
  • Para crear una plataforma escalable para la ingeniería de circuitos de proteínas en células de mamíferos.

Principales métodos:

  • Las proteasas virales diseñadas fueron diseñadas para funcionar como proteasas modulares ortogonales (CHOMP).
  • Las proteasas de entrada fueron diseñadas para acoplarse y dividir las proteasas objetivo, inhibiendo su función.
  • Los componentes de CHOMP se ensamblaron en cascadas reguladoras, puertas lógicas y funciones de procesamiento de señales.

Principales resultados:

  • El sistema CHOMP implementó con éxito diversas funciones a nivel de circuito en células de mamíferos.
  • Un circuito CHOMP diseñado racionalmente indujo la muerte celular en respuesta a la activación del oncogén Ras.
  • Los circuitos CHOMP pueden codificarse como transcripciones individuales y entregarse sin integración genómica.

Conclusiones:

  • Las proteasas virales diseñadas proporcionan una plataforma versátil y compuesta para circuitos de proteínas sintéticas.
  • El sistema CHOMP permite la creación de funciones complejas de regulación y procesamiento de señales.
  • CHOMP ofrece un enfoque escalable para la ingeniería de circuitos de proteínas en biotecnología.