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The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
Published on: April 25, 2016
Participación de vías comunes y específicas del tipo celular en el control del gen c-fos: inducción estable de cAMP
Cell
|January 30, 1987
Resumen
El factor de crecimiento derivado de plaquetas (PDGF) y el factor estimulante de colonias 1 (CSF-1) inducen transitoriamente c-fos en fibroblastos y macrófagos, respectivamente. La AMP cíclica (cAMP) induce una transcripción c-fos estable en los macrófagos, lo que indica mecanismos reguladores distintos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La señalización celular de las células.
- Regulación genética Reglamento genético.
Sus antecedentes:
- El proto-oncogén c-fos es rápidamente inducido por estímulos externos como PDGF y CSF-1.
- La descomposición de los fosfolípidos, la proteína quinasa C (PKC) y la liberación de Ca2+ están implicadas en la inducción de c-fos.
- El papel de la calmodulina en la degradación del ARNm c-fos se sugiere por sus efectos inhibidores.
Objetivo del estudio:
- Investigar los mecanismos que regulan la inducción del protooncogén c-fos en los fibroblastos y los macrófagos.
- Explorar las funciones diferenciales de las vías de señalización, incluyendo PKC, Ca2+, calmodulina y nucleótidos cíclicos, en la expresión génica c-fos.
Principales métodos:
- Cultivo celular de fibroblastos y macrófagos.
- Tratamiento con factores de crecimiento (PDGF, CSF-1) y moduladores de la señalización (inhibidores de la calmodulina, nucleótidos cíclicos).
- Análisis de los niveles de ARNm y proteínas c-fos y las tasas de transcripción génica.
Principales resultados:
- PDGF induce transitoriamente el c-fos en los fibroblastos; CSF-1 induce transitoriamente el c-fos en los macrófagos.
- Las vías de señalización de PKC y Ca2+ están involucradas en la inducción transitoria de c-fos.
- La inhibición de la calmodulina prolonga el ARNm c-fos y la inducción de proteínas por el PDGF.
- cAMP induce fuertemente la transcripción c-fos estable en los macrófagos, a diferencia de los fibroblastos.
Conclusiones:
- Distintas vías de señalización regulan la expresión del proto-oncogén c-fos en respuesta a diferentes estímulos.
- La calmodulina juega un papel en la regulación de la degradación del ARNm c-fos.
- Los macrófagos exhiben una regulación diferencial de la transcripción de c-fos por cAMP en comparación con los fibroblastos, lo que sugiere mecanismos específicos de la célula.
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