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La transformación por el virus del sarcoma de Rous induce un nuevo gen con homología a una proteína plaquetaria
Cell
|May 8, 1987
Resumen
Los investigadores identificaron un gen, 9E3, que está altamente expresado en las células transformadas por el virus del sarcoma de Rous. Este gen codifica una proteína similar al péptido III activador del tejido conectivo, que contribuye potencialmente a las características de las células cancerosas.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Virología.
- Biología celular Biología celular.
Sus antecedentes:
- La transformación del virus del sarcoma de Rous (RSV) de los fibroblastos del embrión de pollo (CEF) conduce a cambios celulares significativos.
- Comprender los mecanismos moleculares detrás de la oncogénesis viral es crucial para identificar objetivos terapéuticos.
Objetivo del estudio:
- Identificar y caracterizar nuevos genes regulados al alza durante la transformación celular inducida por el RSV.
- Para aclarar el papel potencial del producto genético identificado en las alteraciones fenotípicas de las células transformadas.
Principales métodos:
- Aislamiento y caracterización de un clon de ADNc (9E3) de una biblioteca de ADNc del CEF.
- Análisis cuantitativo de los niveles de ARNm 9E3 en FEC normal y transformada por el RSV.
- Secuenciación de nucleótidos y traducción in vitro del ADNc 9E3.
- Comparación de la secuencia polipeptídica deducida con proteínas conocidas.
Principales resultados:
- Se aisló un clon de ADNc, 9E3, y se encontró que estaba significativamente regulado al alza (20 veces) en el CEF transformado por el RSV debido al aumento de la transcripción.
- Se observaron niveles elevados de ARNm 9E3 rápidamente (dentro de 2 horas) después del cambio de temperatura en CEF infectados con mutantes RSV sensibles a la temperatura.
- El ARNm 9E3 codifica un polipéptido de 11 kDa homólogo al péptido activador del tejido conectivo humano III (CTAP-III), la beta-tromboglobulina y el factor plaquetario 4.
Conclusiones:
- El gen 9E3 es rápidamente y altamente inducido durante la transformación RSV de CEF.
- El polipéptido 9E3, que comparte homología con CTAP-III, puede contribuir a las características fenotípicas observadas de las células transformadas por RSV.
- Se justifica una mayor investigación sobre las actividades biológicas de 9E3 para comprender su papel en la transformación celular y el cáncer.
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