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Un represor transcripcional codificado por BPV-1 comparte un dominio carboxiterminal común con el transactivador E2
Cell
|July 3, 1987
Resumen
Los investigadores identificaron un factor regulador del virus del papiloma bovino tipo 1 (BPV-1) que inhibe la transformación celular. Este factor de acción negativa, codificado por el marco de lectura abierto E2, reprime la expresión y transformación de genes virales.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Investigación de Investigación del Cáncer.
Sus antecedentes:
- Se sabe que el virus del papiloma bovino tipo 1 (BPV-1) causa tumores.
- La expresión y regulación de los genes virales son fundamentales para la transformación celular inducida por BPV-1.
Objetivo del estudio:
- Identificar y caracterizar los factores reguladores transcripcionales de acción negativa codificados por BPV-1.
- Para aclarar el mecanismo por el cual BPV-1 regula su propia actividad de transformación.
Principales métodos:
- Identificación de un factor de acción negativa a través de ensayos funcionales.
- Análisis de la transformación de BPV-1 de las células C127 del ratón.
- Expresión basada en plásmidos de las funciones inhibidoras.
- Mapeo de la función inhibidora al dominio 3' del marco de lectura abierto E2.
Principales resultados:
- Se identificó un factor regulador transcripcional de acción negativa codificado por BPV-1.
- Este factor inhibe específicamente la transformación mediada por BPV-1 de las células C127 del ratón.
- El factor suprime la transactivación E2 del potenciador BPV-1 a través de un elemento cis-activo compartido.
- La inhibición de la transformación se correlaciona con la regulación a la baja de la expresión génica viral dependiente de E2.
Conclusiones:
- El marco de lectura abierto E2 de BPV-1 codifica tanto factores reguladores transcripcionales positivos como negativos.
- Estos factores reguladores comparten un dominio carboxiterminal común.
- BPV-1 posee un mecanismo de autorregulación que involucra a su proteína E2 para controlar la transformación.
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