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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
Published on: February 20, 2017
El control del ciclo celular de la levadura está asociado con el ensamblaje/desensamblaje del complejo de proteína
1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell
|September 23, 1988
Resumen
El producto genético de Saccharomyces cerevisiae CDC28, un complejo de proteína quinasa, es esencial para la progresión del ciclo celular. Su ensamblaje y actividad, particularmente la fosforilación de p40, regulan la entrada en la fase S.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El ciclo celular es un proceso fundamental en la división celular eucariota.
- La progresión a través del ciclo celular está estrictamente regulada por las proteínas cinasas.
- El gen de Saccharomyces cerevisiae CDC28 codifica una proteína quinasa clave involucrada en el control del ciclo celular.
Objetivo del estudio:
- Para caracterizar la forma activa de la proteína quinasa Cdc28.
- Para investigar la regulación de la actividad de la cinasa Cdc28 durante el ciclo celular.
- Para aclarar el papel de la fosforilación p40 en la iniciación del ciclo celular.
Principales métodos:
- Análisis bioquímico de complejos proteicos.
- Ensayos in vitro de la quinasa utilizando las histonas H1 y p40.
- Experimentos de sincronización y detención del ciclo celular.
Principales resultados:
- La proteína quinasa activa Cdc28 existe como un complejo de ~ 160 kDa que contiene p40.
- Ensamblaje complejo y p40 pico de fosforilación durante la fase G1.
- La detención del ciclo celular en G1 conduce a la inactivación de la quinasa y el desmontaje complejo.
- Los complejos parciales fosforilan la histona H1 pero requieren factores solubles para la fosforilación p40.
Conclusiones:
- La fosforilación p40 por el complejo Cdc28 es crucial para iniciar un nuevo ciclo celular.
- El ensamblaje y la actividad del complejo cinasa Cdc28 están regulados por el ciclo celular.
- Se propone un modelo donde la fosforilación p40 actúa como un disparador específico de G1 para la iniciación del ciclo celular.
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