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Los nanosensores plasmónicos revelan una dependencia de la altura de las oscilaciones de las proteínas MinDE en las

Weixiang Ye^1,2, Sirin Celiksoy¹, Arpad Jakab¹

¹Institute of Physical Chemistry , University of Mainz , Duesbergweg 10-14 , 55128 Mainz , Germany.

Journal of the American Chemical Society

|November 28, 2018

Resumen

Este estudio mejora la espectroscopia de plasma de una sola partícula para medir las distancias de las macromoléculas de los sensores. El nuevo método revela la dinámica de la proteína Min bacteriana en las membranas lipídicas, influenciada por la composición y la curvatura.

Área de la Ciencia:

La biofísica
Nanotecnología
La bioquímica

Sus antecedentes:

La espectroscopia de plasma de una sola partícula (SPPS) detecta las macromoléculas sin etiqueta.
Los métodos actuales de SPPS carecen de mediciones precisas de la distancia de los sensores.

Objetivo del estudio:

Extender el SPPS para la determinación a distancia subnanométrica de las macromoléculas con sensores plasmónicos.
Investigar la dinámica de interacción de las proteínas Min bacterianas con las membranas lipídicas.

Principales métodos:

SPPS mejorado para registrar simultáneamente espectros de dispersión de miles de nanopartículas de diferentes tamaños.
Se logra una alta resolución de tiempo (1,8 s) y la adquisición continua de datos durante horas.
Tamaño de nanopartícula variado sistemáticamente para ajustar el rango de detección y determinar las distancias de las macromoléculas.

Principales resultados:

Resolución subnanométrica demostrada para las mediciones de la distancia de las macromoléculas.
Reveló los modos de funcionamiento flexibles de las proteínas Min bacterianas en las membranas lipídicas.
Se observa la oscilación de la proteína sobre un parche MinD estacionario en presencia de cardiolipina y curvatura de la membrana.

Conclusiones:

La composición de la membrana y la curvatura local son críticas para comprender el sistema de proteínas Min.
El método SPPS desarrollado sin etiquetas es fácilmente implementable para medir las distancias de las macromoléculas biológicas que interactúan.
Los hallazgos pueden extrapolarse a otros sistemas macromoleculares.

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