Video Experimental Relacionado
Updated: Feb 2, 2026
05:28
Author Spotlight: Unveiling the Molecular Basis of Pain Perception and Neuropathic Pain
Published on: August 9, 2024
1.7K
Imágenes de la red de neuritas con un Aptamer Anti-L1CAM generado por Neurite-SELEX
Linlin Wang1,2, Tao Bing1,2, Ying Liu1,2
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190 , China.
Journal of the American Chemical Society
|November 29, 2018
Resumen
Los investigadores desarrollaron un aptamer de ADN, yly12, que se dirige a las neuritas y su proteína asociada L1CAM. Este aptamer ayuda a visualizar las redes neuronales e inhibe el crecimiento de las neuritas, ofreciendo una nueva herramienta para la investigación en neurociencia.
Área de la Ciencia:
- La neurociencia
- Biotecnología
- Biología molecular
Sus antecedentes:
- El crecimiento de neuritas es crucial para el desarrollo del sistema nervioso y es un factor clave para evaluar la neurotoxicidad y la regeneración nerviosa.
- El desarrollo de sondas moleculares específicas para neuritas es esencial para estas evaluaciones.
Objetivo del estudio:
- Desarrollar una sonda de aptamer de ADN para neuritas utilizando una estrategia SELEX modificada.
- Identificar el objetivo molecular del aptamer y evaluar su utilidad en la investigación neurocientífica.
Principales métodos:
- Se empleó una estrategia SELEX (evolución sistemática de ligandos por enriquecimiento exponencial) específica para neuritas para generar aptameros de ADN.
- Se seleccionó el aptamer yly12 y se identificó su objetivo de unión.
- La eficacia del aptamer se probó en imágenes de células vivas, imágenes de secciones de tejido cerebral y ensayos de inhibición del crecimiento de neuritas.
Principales resultados:
- Se generó un aptamer de ADN, yly12, que se une fuertemente a las neuritas.
- El objetivo molecular de yly12 fue identificado como la molécula de adhesión celular neural L1 (L1CAM).
- yly12 logró obtener imágenes de redes de neuritas en células vivas y tejido cerebral, y demostró un efecto inhibidor en el crecimiento de las neuritas.
Conclusiones:
- El ADN aptamer yly12 es una potente herramienta molecular para la investigación neurocientífica, particularmente para visualizar neuritas y estudiar su desarrollo.
- La estrategia neurita-SELEX dirigida a las estructuras subcelulares es efectiva para la generación de aptamer.
- yly12 tiene potencial para aplicaciones en la investigación de enfermedades neurodegenerativas y el desarrollo de fármacos para la regeneración nerviosa.
Más Videos Relacionados
Videos de Conceptos Relacionados
02:26Protein Networks
4.5K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.5K
02:26Protein Networks
2.9K
2.9K
02:18Network Covalent Solids
16.2K
Network covalent solids contain a three-dimensional network of covalently bonded atoms as found in the crystal structures of nonmetals like diamond, graphite, silicon, and some covalent compounds, such as silicon dioxide (sand) and silicon carbide (carborundum, the abrasive on sandpaper). Many minerals have networks of covalent bonds.
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
16.2K
01:25Network Function of a Circuit
717
Frequency response analysis in electrical circuits provides vital insights into a circuit's behavior as the frequency of the input signal changes. The transfer function, a mathematical tool, is instrumental in understanding this behavior. It defines the relationship between phasor output and input and comes in four types: voltage gain, current gain, transfer impedance, and transfer admittance. The critical components of the transfer function are the poles and zeros.
717
01:22Waterproofing and Anti-Bacterial Admixtures in Concrete
243
Concrete's susceptibility to water absorption is due to the capillary action within the pores of its hydrated cement paste. This action draws water in, creating the need for waterproofing admixtures to prevent such penetration. The efficacy of these admixtures is contingent upon the water pressure, with variations arising from different conditions such as rain, capillary rise, or hydrostatic pressure in structures intended to hold water.
Waterproofing admixtures render concrete hydrophobic,...
Waterproofing admixtures render concrete hydrophobic,...
243
01:24Sequence Networks of Rotating Machines
491
A Y-connected synchronous generator, grounded through a neutral impedance, is designed to produce balanced internal phase voltages with only positive-sequence components. The generator's sequence networks include a source voltage that is exclusively in the positive-sequence network. The sequence components of line-to-ground voltages at the generator terminals illustrate this configuration.
Zero-sequence current induces a voltage drop across the generator's neutral impedance and other...
Zero-sequence current induces a voltage drop across the generator's neutral impedance and other...
491