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Dos dominios conservados de ARN snRNA U2 de levadura están separados por 945 nucleótidos no esenciales
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|October 7, 1988
Resumen
El ARN nuclear pequeño de levadura U2 (ARNn) tiene dominios funcionales distintos. La eliminación de la región central del snRNA U2 de la levadura no tiene ningún efecto en el crecimiento, mientras que alterar el extremo 3' afecta la viabilidad celular.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El ARN nuclear pequeño U2 de la levadura (ARNn) es significativamente más grande que su contraparte en los mamíferos.
- La comprensión de los dominios funcionales de la levadura U2 snRNA es crucial para aclarar su papel en los procesos celulares.
Objetivo del estudio:
- Para investigar la importancia funcional de los diferentes dominios dentro de la gran molécula de levadura U2 snRNA.
- Determinar las regiones esenciales del snRNA U2 de la levadura requeridas para la viabilidad y función celular.
Principales métodos:
- El análisis de eliminación se empleó para eliminar sistemáticamente segmentos de la levadura U2 snRNA.
- El análisis fenotípico, incluida la evaluación de la tasa de crecimiento, se realizó en cepas de levadura con regiones de ARN snU2 eliminadas.
- El análisis de los extremos 3' de U2 snRNA se llevó a cabo en células con deleciones en el dominio terminal 3'.
Principales resultados:
- La molécula de ARN snRNA U2 de la levadura puede dividirse funcionalmente en tres dominios distintos.
- Eliminación del dominio 5' altamente conservado (aprox. 120 nucleótidos) es letal, lo que confirma su papel esencial.
- Sorprendentemente, la eliminación del gran dominio central (945 nucleótidos) no afecta la tasa de crecimiento de la levadura.
- La eliminación de secuencias en el dominio terminal 3' (110 nucleótidos) conduce a un crecimiento lento y a la alteración de los extremos 3' del snRNA U2, lo que sugiere un papel en la estabilidad.
Conclusiones:
- El snRNA U2 de levadura posee al menos dos elementos funcionalmente críticos: el dominio 5' esencial y un dominio 3' involucrado en la formación de partículas.
- El dominio 5' del snRNA U2 de la levadura está directamente implicado en la reacción de empalme.
- El dominio 3 'de la levadura U2 snRNA probablemente juega un papel clave en la generación y la estabilidad de las pequeñas partículas de ribonucleoproteína nuclear (snRNPs).
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