Video Experimental Relacionado
Updated: Feb 1, 2026
09:43
In vivo Near Infrared Fluorescence NIRF Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis
Published on: August 4, 2011
18.6K
Estrategia de diseño molecular para construir la sonda fluorescente de infrarrojo cercano para detectar
Jing Ning1,2, Tao Liu2, Peipei Dong1
1College of Integrative Medicine, The National & Local Joint Engineering Research Center for Drug Development of Neurodegenerative Disease, College of Pharmacy , Dalian Medical University , Dalian 116044 , China.
Journal of the American Chemical Society
|December 12, 2018
Resumen
Los investigadores desarrollaron BnXPI, una nueva sonda fluorescente para el monitoreo en tiempo real de la actividad de la enzima citocromo P450 2J2 (CYP2J2). Esta herramienta mejora la comprensión de CYP2J2
Área de la Ciencia:
- La bioquímica
- Biología molecular
- Biología Química
Sus antecedentes:
- El citocromo P450 2J2 (CYP2J2) es crucial para metabolizar los xenobióticos y los compuestos endógenos, afectando a varios procesos fisiológicos y patológicos.
- El papel de CYP2J2 en la tumorigénesis y el diagnóstico del cáncer es poco conocido debido a la falta de herramientas de monitoreo en tiempo real.
Objetivo del estudio:
- Desarrollar una herramienta molecular para el monitoreo en tiempo real de la actividad del CYP2J2 en sistemas biológicos.
- Investigar las funciones biológicas del CYP2J2 en la tumorigénesis.
Principales métodos:
- Se utilizaron principios de diseño molecular para crear una sonda fluorescente selectiva de CYP2J2 (HXPI) utilizando un fluoróforo de infrarrojo cercano.
- Se incorporó un vinculante autoinmolador a los derivados de HXPI para mejorar la reactividad y la especificidad del canal estrecho de sustrato del CYP2J2.
- BnXPI, un derivado de HXPI O-alquilado, fue identificado como la sonda óptima después del cribado.
Principales resultados:
- BnXPI demostró una alta especificidad, sensibilidad y aplicabilidad para la detección de CYP2J2 in vitro e in vivo.
- Tras la O- desmetilación por el CYP2J2, BnXPI sufre una reacción autoinmoladora, liberando HXPI y permitiendo el monitoreo de la fluorescencia en tiempo real.
- BnXPI monitoreó con éxito la actividad de CYP2J2 en sistemas vivos, incluidas las células, los tejidos tumorales y los animales portadores de tumores.
Conclusiones:
- Se estableció una estrategia práctica para desarrollar herramientas altamente específicas y de amplia aplicación para el monitoreo del CYP2J2.
- BnXPI ofrece una herramienta prometedora para explorar las funciones biológicas de CYP2J2 en la tumorigénesis y el diagnóstico del cáncer.
Videos de Conceptos Relacionados
01:13Design Example: Dimensioning of Concrete Masonry Construction
306
For the construction of a storeroom using concrete masonry units, it's essential to align the dimensions of the structure with the actual sizes of the blocks and the intended mortar joints. On the site in question, there's a stockpile of concrete masonry blocks with a nominal size of eight by eight by sixteen inches, which are to be used in the construction of the storeroom.
The site engineer has laid out a plan for the storeroom with external dimensions of twelve feet in length and...
The site engineer has laid out a plan for the storeroom with external dimensions of twelve feet in length and...
306
02:57The Sense of Self: Reflected Self-Appraisal and Social Comparison
56.1K
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
56.1K
02:35Molecular Orbital Theory I
47.5K
Overview of Molecular Orbital Theory
47.5K
02:01Group Design
10.5K
The most basic experimental design involves two groups: the experimental group and the control group. The two groups are designed to be the same except for one difference— experimental manipulation. The experimental group gets the experimental manipulation—that is, the treatment or variable being tested—and the control group does not. Since experimental manipulation is the only difference between the experimental and control groups, we can be sure that any differences between...
10.5K
01:26Introduction to Special Senses
7.5K
Sensory receptors play an integral part in comprehending our external and internal environments. They receive diverse stimuli, converting them into the nervous system's electrochemical signals. This conversion occurs as the stimulus alters the sensory neuron's cell membrane potential, instigating the generation of an action potential. This action potential is subsequently transmitted to the central nervous system (CNS), which integrates with other sensory data or higher cognitive...
7.5K
01:27Tactile and Chemical Senses
796
Tactile senses encompass touch, temperature, and pain, each mediated by specific receptors. Touch receptors detect mechanical energy or pressure against the skin. Sensory fibers from these receptors enter the spinal cord and relay information to the brain stem. Here, most fibers cross over to the opposite side of the brain. The touch information then moves to the thalamus, which projects a map of the body's surface onto the somatosensory areas of the parietal lobes in the cerebral cortex.
796