Video Experimental Relacionado

Updated: Jan 31, 2026

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT

Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT

Published on: January 7, 2018

La miocina/ cardiocina sensible a la glucosa MG53 regula la respuesta sistémica a la insulina y la homeostasis

Hong-Kun Wu¹, Yan Zhang¹, Chun-Mei Cao¹

¹State Key Laboratory of Membrane Biology, Institute of Molecular Medicine (H.-K.W., Y.Z., C.-M.C., X.H., M.F., Y.Y., L.J., G.C., P.J., S.Z., R.S., W.P., F.L., J.G., L.T., Y.H., D.S., Z.Z., F.L., R.-P.X.).

|December 28, 2018

Resumen

Mitsugumin 53 (MG53) es secretado por el músculo y el corazón en respuesta a la glucosa alta, causando resistencia a la insulina. La neutralización de MG53 ofrece una terapia potencial para la diabetes mellitus tipo 2 (DMT2).

Área de la Ciencia:

Endocrinología
Investigación del síndrome metabólico
Fisiología muscular

Sus antecedentes:

La mitsugumina 53 (MG53), una ligasa E3 específica del músculo, degrada los receptores de insulina, lo que contribuye a la resistencia a la insulina, el síndrome metabólico y la diabetes mellitus tipo 2 (DMT2).
El papel sistémico de MG53 en la regulación de la sensibilidad a la insulina y el metabolismo energético sigue sin estar claro, a pesar de la evidencia de que las miocinas secretadas por el músculo influyen en los procesos sistémicos.

Objetivo del estudio:

Investigar si MG53 funciona como una miocina/cardiocina que regula la sensibilidad sistémica a la insulina y la homeostasis metabólica.
Explorar el potencial de dirigirse al MG53 circulante como estrategia terapéutica para la DM2.

Principales métodos:

Concentración evaluada de proteína MG53 en perfusados de corazón/músculo esquelético de roedores bajo condiciones de glucosa/insulina altas.
Se han medido los niveles séricos de MG53 en roedores y humanos con o sin DM2.
Efectos evaluados de la administración sistémica de MG53 y la neutralización de anticuerpos en modelos de ratón.
Se analizó la interacción de MG53 con el dominio extracelular del receptor de insulina.

Principales resultados:

Los niveles altos de glucosa/insulina estimulan la secreción de MG53 desde el corazón y el músculo esquelético.
Los niveles circulantes de MG53 están elevados en humanos y roedores diabéticos.
La administración sistémica de MG53 induce resistencia a la insulina y síndrome metabólico; la neutralización de MG53 muestra efectos terapéuticos en ratones con DM2.
MG53 se une al dominio extracelular del receptor de insulina, actuando como un bloqueador alostérico.

Conclusiones:

MG53 actúa como una miocina/ cardiocina sensible a la glucosa, exacerbando la resistencia a la insulina mediante la inhibición de la señalización de la insulina.
La orientación al MG53 circulante presenta un nuevo enfoque terapéutico para la DM2 y sus complicaciones.

Palabras clave:

MG53 (en inglés)Diabetes mellitus de tipo 2 trastornos del metabolismo de la glucosa Resistencia a la insulina Síndrome metabólico Obesidad el receptor de la insulina

Más Videos Relacionados

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Published on: June 25, 2017

Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test

Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test

Published on: November 13, 2016

Videos de Conceptos Relacionados

Glucose Homeostasis: Regulation of Blood Glucose

Glucose Homeostasis: Regulation of Blood Glucose

Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion

The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...

Regulation of Metabolism

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

Hormones Regulating Blood Glucose

Hormones Regulating Blood Glucose

Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...

What is Homeostasis?

What is Homeostasis?

Maintaining homeostasis requires that the body continuously maintain its internal conditions. Each physiological condition has a particular set point, from body temperature to blood pressure to levels of certain nutrients. A set point is the physiological value around which the normal range fluctuates. A normal range is a restricted set of values that is optimally healthful and stable. For example, the set point for normal human body temperature is approximately 37°C (98.6°F).

pH Homeostasis

pH Homeostasis

Acid-base homeostasis is essential for maintaining normal physiological activities in humans. The pH of various body fluids is strictly regulated because it is critical for the optimal activity of enzymes involved in metabolic reactions. Enzymes are basically proteins, so, any significant change in pH can affect their structure and activity. In humans, pH is regulated using three primary mechanisms— chemical buffer systems, respiratory regulation, and renal regulation.
Respiratory...