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Fibrillas amiloides de proteínas de apoferritina con quiralidad ajustable y polimorfismo

Rocío Jurado¹, Jozef Adamcik², Miguel López-Haro³

¹Department of Inorganic Chemistry , University of Granada , 18071 Granada , Spain.

Journal of the American Chemical Society

|December 28, 2018

Resumen

La apoferritina recombinante humana puede formar fibrillas amiloides, similares a las de las enfermedades neurodegenerativas. Este estudio revela un vínculo entre la estructura de la apoferritina, la quiralidad y los roles potenciales en las patologías relacionadas con la ferritina.

Área de la Ciencia:

La bioquímica
La neurociencia
Ciencias de los materiales

Sus antecedentes:

La ferritina es vital para el metabolismo y la regulación del hierro en el cerebro.
El mal plegamiento de proteínas está implicado en trastornos neurodegenerativos como el Alzheimer y el Parkinson.
La apoferritina, el caparazón proteico de la ferritina, es un candidato potencial para cambios estructurales patológicos.

Objetivo del estudio:

Investigar la transformación estructural de la apoferritina recombinante humana.
Caracterizar las fibrillas amiloideas resultantes y sus propiedades.
Explorar la conexión entre la estructura de la apoferritina, la quiralidad y los posibles mecanismos de la enfermedad.

Principales métodos:

Técnicas avanzadas de microscopía
Métodos de espectroscopia
Técnicas de dispersión

Principales resultados:

La apoferritina recombinante humana se convierte en fibrillas amiloides.
Las fibrillas de apoferritina presentan una estructura de cinta retorcida de doble cadena.
Se identificó una correlación directa entre la quiralidad de las fibrillas, la morfología y la composición de las subunidades.

Conclusiones:

La apoferritina puede formar estructuras amiloides, lo que sugiere un papel en las patologías relacionadas con la ferritina.
El estudio proporciona información sobre la base estructural del mal plegamiento de la apoferritina.
Los hallazgos abren vías para el diseño de nanoestructuras quirales basadas en el autoensamblaje de proteínas.

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