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Comunalidad a pesar de la excepcional diversidad en el repertorio de anticuerpos humanos de referencia

Bryan Briney^1,2,3,4,5, Anne Inderbitzin^6,7, Collin Joyce^6,8,9,10

¹Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA. briney@scripps.edu.

|January 22, 2019

Resumen

El repertorio de anticuerpos humanos es excepcionalmente diverso, con billones de anticuerpos únicos. Este estudio analizó casi 3 mil millones de secuencias de anticuerpos, revelando la singularidad individual y las características compartidas de anticuerpos en el sistema inmunológico adaptativo.

Área de la Ciencia:

Inmunología
La genómica
La bioinformática

Sus antecedentes:

El sistema inmunológico adaptativo humano genera un vasto repertorio de anticuerpos para reconocer diversos antígenos.
La inmensa diversidad de repertorios de anticuerpos ha limitado históricamente el estudio exhaustivo.
La investigación actual a menudo se centra en las células B circulantes debido a la accesibilidad.

Objetivo del estudio:

Para analizar el repertorio de anticuerpos humanos en una escala sin precedentes.
Investigar la diversidad y las características de las poblaciones de células B circulantes.
Proporcionar un gran conjunto de datos para estudios genéticos del repertorio de anticuerpos.

Principales métodos:

Análisis de casi 3 mil millones de secuencias de cadena pesada de anticuerpos de diez sujetos humanos.
Centrarse en las poblaciones de células B circulantes.
Secuenciación a gran escala y análisis de datos de genes receptores inmunes adaptativos.

Principales resultados:

El estudio presenta la mayor colección de secuencias de receptores inmunes adaptativos hasta la fecha.
Se encontró que los repertorios de anticuerpos son en gran medida únicos para cada individuo.
Se identificó una subpoblación de anticuerpos clonados universalmente compartidos.
Se confirmó una diversidad general excepcional del repertorio de anticuerpos humanos.

Conclusiones:

El repertorio de anticuerpos humanos muestra una notable diversidad y especificidad individual.
Los hallazgos proporcionan un conjunto de datos fundamentales para comprender la genética del repertorio de anticuerpos de referencia.
Las investigaciones adicionales pueden explorar las implicaciones de los clonotipos de anticuerpos compartidos y únicos.

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