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La proteína del centrosoma AKNA regula la neurogénesis a través de la organización de los microtúbulos
Germán Camargo Ortega1,2,3, Sven Falk1,2, Pia A Johansson1,2,4
1Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany.
Nature
|February 22, 2019
Resumen
La proteína del centrosoma AKNA organiza los microtúbulos para controlar la delaminación de las células madre neurales y la salida de la zona subventricular durante el desarrollo del cerebro. AKNA es crucial para regular el comportamiento celular en el cerebro en desarrollo.
Área de la Ciencia:
- La neurociencia
- Biología celular
- Biología del desarrollo
Sus antecedentes:
- La expansión del tamaño del cerebro se correlaciona con el agrandamiento de la zona subventricular durante el desarrollo.
- Las células madre neurales en la zona ventricular generan progenitores basales que se delaminan en la zona subventricular.
- El tiempo de residencia en la zona subventricular es crítico para la amplificación celular y la determinación del destino.
Objetivo del estudio:
- Investigar el papel de la proteína del centrosoma interfase AKNA en la regulación del comportamiento de las células madre neurales.
- Para aclarar la función de AKNA en la formación de la zona subventricular y la delaminación celular.
Principales métodos:
- Inmunolocalización de AKNA en células madre neurales y progenitores basales.
- Evaluación de la necesidad y la suficiencia de AKNA para organizar los microtúbulos centrosomales.
- Análisis del papel de AKNA en la delaminación celular y la salida de la zona subventricular.
Principales resultados:
- AKNA se localiza en los apéndices subdistal de la madre centríola en células madre neurales específicas y progenitores basales.
- AKNA es esencial y suficiente para organizar los microtúbulos centrosomales, promoviendo la nucleación y el crecimiento.
- AKNA media la delaminación durante la formación de la zona subventricular y regula la salida de esta zona.
Conclusiones:
- AKNA juega un papel fundamental en la organización de microtúbulos centrosomales, esencial para que las células madre neurales entren y permanezcan en la zona subventricular.
- La regulación de la delaminación celular por el AKNA es importante para la formación de la zona subventricular y el desarrollo del cerebro.
- AKNA también regula la transición epitelial a mesenquimal en otros tipos de células, lo que indica un papel general en el control de la delaminación celular.
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