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Base estructural para la recuperación dependiente del pH de las proteínas ER del Golgi por el receptor KDEL
Philipp Bräuer1, Joanne L Parker1, Andreas Gerondopoulos1
1Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Science (New York, N.Y.)
|March 9, 2019
Resumen
Este estudio revela la estructura del receptor KDEL, crucial para recuperar las proteínas del retículo endoplasmático (ER) del aparato de Golgi. Su mecanismo similar al transportador y sus interacciones dependientes del pH aseguran una recuperación eficiente de proteínas en las células eucariotas.
Área de la Ciencia:
- Biología celular
- Biología estructural
- La bioquímica
Sus antecedentes:
- El transporte selectivo de proteínas entre el retículo endoplasmático (ER) y Golgi es vital para las células eucariotas.
- La recuperación de las proteínas luminales ER del Golgi se basa en el reconocimiento dependiente del pH de la señal Lys-Asp-Glu-Leu (KDEL) por el receptor KDEL.
Objetivo del estudio:
- Aclarar la base estructural del reconocimiento de la señal KDEL dependiente del pH por el receptor KDEL.
- Comprender los cambios conformacionales involucrados en la recuperación de proteínas dentro de la vía secretora.
Principales métodos:
- Se utilizó la cristalografía de rayos X para determinar las estructuras del receptor KDEL de pollo en diferentes estados (apo ER, Golgi ligado a KDEL y complejo de sybody).
- Se realizaron ensayos de unión in vitro y estudios de localización celular in vivo.
Principales resultados:
- Las estructuras cristalinas revelaron una arquitectura similar al transportador del receptor KDEL.
- La unión de KDEL induce cambios conformacionales significativos.
- Se identificó una red de interacción dependiente del pH crucial para el reconocimiento de la señal KDEL.
Conclusiones:
- El receptor KDEL funciona como un transportador dependiente del pH, facilitando la recuperación de las proteínas ER.
- Las ideas estructurales explican el mecanismo del sistema de recuperación dependiente del pH en la vía secretora.
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