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Los exones 5' alternativos y la expresión específica del tejido de las transcripciones del receptor homólogo del FEA

E D Schejter, D Segal, L Glazer

|September 26, 1986

Resumen

Los investigadores secuenciaron el gen homólogo del receptor del factor de crecimiento epidérmico (DER) de Drosophila, revelando un único tercer dominio extracelular y N-terminos alternativos. Las transcripciones de DER se encuentran en los tejidos en desarrollo y adultos de Drosophila, particularmente en las células proliferantes y el sistema nervioso.

Área de la Ciencia:

Biología del desarrollo Biología del desarrollo.
Biología Molecular Biología Molecular
Genética La genética.

Sus antecedentes:

La familia de receptores del factor de crecimiento epidérmico (EGFR) juega un papel crucial en el crecimiento, la diferenciación y el desarrollo celular.
La comprensión de los homólogos de los receptores del factor de crecimiento humano en organismos modelo como Drosophila puede proporcionar información sobre las funciones biológicas conservadas.

Objetivo del estudio:

Para aislar y caracterizar el cDNA de la Drosophila homólogo del receptor del factor de crecimiento epidérmico (DER) del gen.
Investigar las características estructurales y los patrones de expresión de DER durante el desarrollo de Drosophila.

Principales métodos:

clonación y secuenciación de ADNc para determinar la estructura del gen y deducir las secuencias de aminoácidos.
Hibridación in situ para analizar la distribución espacial y temporal de las transcripciones de DER en embriones, larvas y adultos.

Principales resultados:

El gen DER codifica una proteína con homología significativa a las proteínas humanas EGFR y neu, con un dominio extracelular adicional rico en cisteína.
El empalme alternativo del gen DER da como resultado tres isoformas de proteínas distintas que difieren en sus N-terminales.
Las transcripciones de DER se expresan ubicuamente en los embriones, con la expresión larval concentrada en los discos imaginarios y la corteza cerebral, y la expresión adulta principalmente en el cerebro y los ganglios.

Conclusiones:

El gen DER es un homólogo del receptor del factor de crecimiento epidérmico de mamíferos, exhibiendo características estructurales únicas.
El empalme alternativo genera múltiples variantes de la proteína DER, lo que sugiere complejos mecanismos reguladores.
El patrón de expresión del desarrollo de DER indica su participación en la proliferación celular y el desarrollo neuronal en Drosophila.

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