Video Experimental Relacionado
Updated: Jan 26, 2026
08:22
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
Published on: March 23, 2022
6.5K
Las células linfoides innatas apoyan a las células T reguladoras en el intestino a través de la interleucina-2
Lei Zhou1,2,3, Coco Chu1,2,3, Fei Teng1,2,3
1Division of Gastroenterology, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, Cornell University, New York, NY, USA.
Nature
|April 5, 2019
Resumen
La interleucina-2 (IL-2) es crucial para la inmunidad intestinal. Las células linfoides innatas del grupo 3 (ILC3) producen IL-2, que mantiene las células T reguladoras (Treg) y previene la inflamación, una vía deteriorada en la enfermedad de Crohn.
Área de la Ciencia:
- Inmunología
- Gastroenterología
- Investigación del microbioma
Sus antecedentes:
- La interleucina-2 (IL-2) es vital para prevenir la inflamación gastrointestinal mediante el apoyo a las células T (Treg) reguladoras.
- La terapia con dosis bajas de IL-2 es un tratamiento potencial para la enfermedad inflamatoria intestinal.
- Las fuentes celulares y moleculares de IL-2 en el intestino siguen sin estar claras.
Objetivo del estudio:
- Elucidar las fuentes celulares y las vías reguladoras de la producción de IL-2 en el intestino delgado.
- Investigar el papel de la IL-2 en el mantenimiento de la homeostasis inmune intestinal y la función de las células Treg.
- Para explorar la relevancia de esta vía en la enfermedad inflamatoria intestinal humana.
Principales métodos:
- Utilizó un modelo de ratón con eliminación de genes específicos del linaje para rastrear la producción de IL-2.
- Se utilizaron análisis imparciales para identificar las células productoras de IL-2 en el intestino delgado.
- Investigó el papel de IL-1β, los macrófagos, MYD88 y NOD2 en la regulación de la producción de IL-2.
- Se evaluaron los niveles de células IL-2 y Treg en tejidos del intestino delgado de pacientes con enfermedad de Crohn.
Principales resultados:
- Las células linfoides innatas del grupo 3 (ILC3) son la fuente primaria de IL-2 en el intestino delgado.
- La producción de IL-2 por ILC3s es inducida por IL-1β, que es producido por macrófagos que detectan la microbiota a través de MYD88 y NOD2.
- La IL-2 derivada de ILC3 es esencial para el mantenimiento de las células Treg, la homeostasis inmune y la tolerancia oral.
- Se observó una reducción de la producción de IL-2 por ILC3 y frecuencias más bajas de células Treg en pacientes con enfermedad de Crohn.
Conclusiones:
- Una nueva vía que involucra microbiota, macrófagos, IL-1β e ILC3 regula la producción de IL-2 en el intestino delgado.
- La IL-2 derivada de ILC3 juega un papel crítico en la regulación inmune intestinal y la tolerancia oral.
- La desregulación de esta vía IL-2 en ILC3 puede contribuir a la patogénesis de la enfermedad de Crohn.
Videos de Conceptos Relacionados
01:18Lymphoid Cells and Tissues
3.0K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
3.0K
01:28Cells of the Innate Immune Response
8.8K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.8K
02:02Cis-regulatory Sequences
11.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
01:23Renewal of Intestinal Stem Cells
3.2K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.2K
01:23Anatomy of the Intestines
87.2K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
87.2K
01:15Secondary Lymphoid Organs
9.0K
Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
9.0K