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El paisaje emergente del endodermo intestinal del ratón en resolución de una sola célula
Sonja Nowotschin1, Manu Setty2, Ying-Yi Kuo1
1Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Nature
|April 9, 2019
Resumen
Este estudio mapea el desarrollo del endodermo de los mamíferos utilizando transcriptomas de una sola célula, revelando cómo las células epiblásticas forman el endodermo en dos momentos clave y cómo estas células modelan el tubo intestinal temprano.
Área de la Ciencia:
- Biología del desarrollo
- La genómica
- Biología celular
Sus antecedentes:
- El desarrollo temprano del endodermo de los mamíferos es crucial para la organogénesis.
- La comprensión de la ontogenia endodérmica requiere un mapeo detallado de la diferenciación celular y las trayectorias de linaje.
Objetivo del estudio:
- Delinear la ontogenia del endodermo de los mamíferos desde el desarrollo temprano hasta la gestación media.
- Caracterizar la dinámica espacio-temporal y la arquitectura transcripcional del desarrollo del endodermo.
- Descubrir la relación entre los linajes de epiblastos y endodermos durante el desarrollo.
Principales métodos:
- Generación y análisis de 112.217 transcriptomas unicelulares de embriones de ratón.
- Aplicación de enfoques basados en gráficos para modelar la diferenciación celular y las trayectorias de desarrollo.
- Mapeo espacio-temporal de los destinos celulares del endodermo y el patrón del tubo intestinal.
Principales resultados:
- Definido el paisaje transcripcional de todas las poblaciones de endodermo hasta la gestación media.
- Se identificaron dos puntos de tiempo distintos para la diferenciación del epiblasto al endodermo (antes y durante la gastrulación).
- Reveló características conservadas pero específicas del linaje en las células endoderma que convergen en el tubo intestinal.
- Identidad celular regionalizada observada a lo largo del eje anterior-posterior y patrón específico de órgano coordinado.
Conclusiones:
- El desarrollo del endodermo de los mamíferos se caracteriza por ondas de diferenciación distintas y contribuciones de linaje.
- El tubo intestinal emergente exhibe un patrón espacial que refleja los orígenes embrionarios / extraembrionarios y la historia del linaje.
- Este estudio proporciona un atlas transcripcional completo del desarrollo temprano del endodermo y la formación del tubo intestinal.
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