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Inducibilidad de la proteína de unión al potenciador de inmunoglobulina kappa Nf-kappa B por un mecanismo
Cell
|December 26, 1986
Resumen
El factor nuclear kappa B (NF-kappa B) es activado por la modificación post-traduccional. Esta proteína es la proteína
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Inmunología Inmunología.
- Biología celular Biología celular.
Sus antecedentes:
- El factor nuclear kappa B (NF-kappa B) es una proteína nuclear que interactúa con el potenciador de inmunoglobulina kappa.
- El NF-kappa B se encuentra en las células que transcriben los genes de la cadena ligera de inmunoglobulinas y puede ser inducido en las células pre-B por el lipopolisacárido (LPS).
Objetivo del estudio:
- Investigar los mecanismos de activación y la distribución celular de NF-kappa B. para investigar los mecanismos de activación y la distribución celular de NF-kappa B. para investigar los mecanismos de activación y la distribución celular de NF-kappa B. para investigar los mecanismos de activación y la distribución celular de NF-kappa B. para investigar los mecanismos de activación y la distribución celular de NF-kappa B. para investigar los mecanismos de activación y la distribución celular de NF-kappa B.
- Para determinar si la inducción de NF-kappa B está restringida a las células linfoides B.
Principales métodos:
- Estimulación de las células pre-B con lipopolisacárido bacteriano (LPS) y cicloheximida.
- Inducción de NF-kappa B utilizando un éster activo de forbol en varias líneas celulares (células T de Jurkat, células HeLa no linfoides).
Principales resultados:
- La estimulación LPS induce NF-kappa B a través de la activación post-traducional en las células pre-B.
- La superinducción de NF-kappa B ocurre con el tratamiento combinado de LPS y cicloheximida.
- El éster de Phorbol induce rápidamente la NF-kappa B en las células T y las células no linfoides, lo que indica una distribución más amplia.
Conclusiones:
- La activación de NF-kappa B no se limita a las células linfoides B.
- Los factores de transcripción que controlan la expresión génica específica pueden ser activados por la modificación post-traducional de factores precursores ampliamente presentes.
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