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Montaje y desmontaje impulsado por enzimas de nanotubos híbridos de ADN-ARN

Siddharth Agarwal¹, Elisa Franco¹

¹Department of Mechanical Engineering , University of California at Riverside , Riverside , California 90095 , United States.

Journal of the American Chemical Society

|May 3, 2019

Resumen

Los investigadores crearon un sistema dinámico de nanotubos de ADN y ARN. Este sistema utiliza programas de transcripción para el ensamblaje y desmontaje controlados a temperatura corporal, inspirados en procesos celulares.

Área de la Ciencia:

Biotecnología
Biología sintética
Nanotecnología

Sus antecedentes:

Las células vivas regulan dinámicamente estructuras como el citoesqueleto utilizando la activación del precursor.
La nanotecnología de ADN sintético ha creado conjuntos complejos, pero carece de control temporal dinámico sobre la formación.
El control del montaje y desmontaje de las nanoestructuras sintéticas sigue siendo un desafío importante.

Objetivo del estudio:

Desarrollar un sistema de nanotubos de ADN-ARN con montaje y desmontaje controlados temporalmente.
Para imitar la capacidad de los sistemas biológicos para controlar conjuntos dinámicos utilizando programas de transcripción.
Para crear nanoestructuras híbridas de ADN-ARN que puedan ensamblarse bajo demanda.

Principales métodos:

Desarrolló un sistema híbrido de nanotubos de ADN y ARN.
Utilizó programas de transcripción para controlar el montaje y desmontaje de nanotubos.
Se emplean enzimas para impulsar la producción y degradación de ARN para el control dinámico.
Se investigó el efecto de los parámetros de reacción en la velocidad de montaje/desmontaje.

Principales resultados:

Control temporal del montaje y desmontaje de los nanotubos a temperatura fisiológica.
Demostró que las moléculas de ARN activan selectivamente los monómeros de ADN inertes para el ensamblaje híbrido.
Mostró el control sintonizable sobre la velocidad de formación y descomposición de nanotubos.
Confirmó que las reacciones enzimáticas impulsan los programas moleculares para el control dinámico.

Conclusiones:

El sistema de nanotubos de ADN-ARN desarrollado ofrece un montaje y desmontaje controlados temporalmente.
Este sistema híbrido está inspirado en los mecanismos biológicos para la formación de estructuras dinámicas.
Las aplicaciones potenciales incluyen la construcción de circuitos biológicos integrados y andamios funcionales en células.
El ensamblaje impulsado por ARN proporciona un mecanismo para la construcción bajo demanda de componentes de ácido nucleico.

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