Video Experimental Relacionado
Updated: Jan 24, 2026
08:52
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
Published on: July 26, 2019
8.5K
Anticorpos para una interfaz de cabeza de gripe conservada Epitopo Proteger por un mecanismo dependiente del subtipo
Akiko Watanabe1, Kevin R McCarthy2, Masayuki Kuraoka1
1Department of Immunology, Duke University, Durham, NC 27710, USA.
Cell
|May 18, 2019
Resumen
Los investigadores identificaron un nuevo epítopo de hemaglutinina (HA) de la gripe conservado que es inmunogénico en los humanos. Los anticuerpos dirigidos a este sitio ofrecen protección en ratones, lo que sugiere la posibilidad de vacunas universales contra la gripe.
Área de la Ciencia:
- Inmunología
- Virología
- Biología estructural
Sus antecedentes:
- La inmunidad humoral duradera contra patógenos en evolución como la gripe requiere anticuerpos dirigidos a epítopos conservados.
- La hemaglutinina de la gripe (HA) es un objetivo clave para neutralizar los anticuerpos, pero su variabilidad antigénica plantea un desafío para el desarrollo de vacunas.
Objetivo del estudio:
- Identificar y caracterizar epítopos nuevos, conservados e inmunogénicos en la hemaglutinina de la gripe (HA).
- Evaluar el potencial protector de los anticuerpos dirigidos a este epitopo recién identificado contra la infección por influenza.
Principales métodos:
- Análisis de células B de memoria individuales de donantes humanos inmunizados.
- Análisis estructural de las interacciones entre la proteína hemaglutinina (HA) de la influenza y los anticuerpos.
- Estudios in vitro e in vivo con anticuerpos y virus de la gripe en modelos de ratón.
Principales resultados:
- Se identificó un epítopo no reconocido previamente en la influenza HA, que es inmunogénico en humanos y se conserva en todos los subtipos.
- Los anticuerpos dirigidos a este epítopo no bloquean la infección viral in vitro, pero proporcionan una protección significativa en ratones tras la administración pasiva.
- El epítopo es conformacionalmente dinámico, expuesto a través de la respiración molecular reversible del trímero de HA.
Conclusiones:
- Este epítopo de HA conservado representa un objetivo prometedor para el desarrollo de vacunas contra la gripe de protección amplia o universal.
- Dirigirse a epítopos conformacionalmente dinámicos podría ser una estrategia viable para obtener inmunidad humoral duradera contra virus que evolucionan rápidamente.
Más Videos Relacionados
13:12Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Published on: April 4, 2011
18.7K
12:09Measuring Influenza Neutralizing Antibody Responses to AH3N2 Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
Published on: November 22, 2017
18.8K
Videos de Conceptos Relacionados
01:05Conservation of Mechanical Energy
24.9K
The mechanical energy E of a system is the sum of its potential energy U and the kinetic energy K of the objects within it. What happens to this mechanical energy when only conservative forces cause energy transfers within the system—that is, when frictional and drag forces do not act on the objects in the system? Also assume that the system is isolated from its environment; in other words no external force from an object outside the system causes energy changes inside the system.
When a...
When a...
24.9K
01:57What is Conservation Biology?
24.0K
Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
24.0K
02:04Conservation of Small Populations
16.7K
Small population sizes put a species at extreme risk of extinction due to a lack of variation, and a consequent decrease in adaptability. This weakens the chances of survival under pressures such as climate change, competition from other species, or new diseases. Large populations are more likely to survive pressures such as these, as such populations are more likely to harbor individuals that have genetic variants that are adaptive under new stresses. Small populations are much less...
16.7K
01:15Transcytosis of IgG
4.1K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
4.1K
01:49Conserved Binding Sites
5.1K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.1K
02:04Protein-protein Interfaces
14.6K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.6K