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Tetrafeniletileno endo y exo-funcionalizado M12L24 Nanosferas: Emisión de fluorescencia dentro de un espacio
Xuzhou Yan1,2, Peifa Wei2, Yuhang Liu1
1School of Chemistry and Chemical Engineering , Shanghai Jiao Tong University , Shanghai 200240 , P. R. China.
Journal of the American Chemical Society
|May 25, 2019
Resumen
Los investigadores crearon materiales artificiales similares a las proteínas fluorescentes verdes (GFP) utilizando nanoesferas de tetrafeniletileno (TPE). Estas nanosferas de TPE imitan el GFP
Área de la Ciencia:
- Química supramolecular
- Ciencias de los materiales
- Química biomimética
Sus antecedentes:
- La proteína fluorescente verde (GFP) y sus propiedades emisoras de luz inspiran estudios de biomimética.
- Imitar el microambiente confinado del barril β de GFP y su "encendido" de fluorescencia es un desafío.
Objetivo del estudio:
- Construir nanoesferas basadas en tetrafeniletileno (TPE) que imiten las propiedades de fluorescencia del GFP.
- Investigar cómo la funcionalidad y el confinamiento de las unidades de TPE afectan la emisión de fluorescencia.
Principales métodos:
- El autoensamblaje impulsado por la coordinación se utilizó para crear nanosferas M12L24 funcionalizadas con TPE.
- Se sintetizaron dos tipos de nanosferas: exofuncionalizadas y endofuncionalizadas.
Principales resultados:
- Ambos tipos de nanosferas de TPE exhibieron una fluorescencia más fuerte que los componentes individuales de TPE en condiciones diluidas.
- Las nanosferas endofuncionalizadas mostraron una emisión significativamente mejorada en comparación con las exofuncionalizadas.
- El movimiento restringido y la mayor concentración local de unidades de TPE dentro de las esferas endofuncionalizadas condujeron a una fluorescencia más fuerte.
Conclusiones:
- El estudio presenta un nuevo enfoque biomimético que utiliza complejos de coordinación supramolecular para crear materiales artificiales similares al GFP.
- Esta metodología ofrece información sobre la construcción de materiales fluorescentes avanzados mediante el control del confinamiento y el movimiento molecular.
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