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Sitio común de unión al ADN para los complejos de proteínas Fos y el factor de transcripción AP-1

F J Rauscher¹, L C Sambucetti, T Curran

¹Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.

|February 12, 1988

Resumen

El complejo de proteína Fos se une al elemento regulador del gen del adipocito P2 (FSE2), con niveles de unión directamente correlacionados con la expresión de Fos. Este sitio de unión también es reconocido por el factor de transcripción AP-1, lo que sugiere un vínculo funcional.

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología celular Biología celular.
Genética La genética.

Sus antecedentes:

El gen del adipocito P2 (aP2) es crucial para la diferenciación de los adipocitos.
Un elemento regulador, FSE2, dentro del gen aP2 se une a un complejo proteico que incluye el producto del protooncogén Fos.
El papel preciso de Fos en la regulación de la unión de FSE2 durante la diferenciación requiere más aclaración.

Objetivo del estudio:

Investigar la relación entre los niveles de expresión de Fos y el complejo de unión FSE2.
Para identificar la secuencia específica de ADN reconocida por el complejo que contiene Fos en FSE2.2.
Explorar la conexión funcional entre Fos y el factor de transcripción AP-1.

Principales métodos:

Análisis del complejo de unión FSE2 en células con expresión Fos variable.
Análisis de inmunoblotación de complejos ADN-proteína.
Análisis mutacional de la secuencia FSE2.
Ensayos de competencia de unión al ADN. ensayos de competencia de unión al ADN.
Foto-enlace cruzado y inmunoprecipitación utilizando anticuerpos contra Fos y Jun.

Principales resultados:

Los niveles complejos de unión de FSE2 reflejan cuantitativa y cualitativamente la expresión de Fos.
La inducción de la expresión de Fos aumenta significativamente el complejo de unión FSE2.
El complejo Fos reconoce la secuencia TGACTCA, un sitio de unión conocido para AP-1 y GCN4.
Las proteínas relacionadas con AP-1 y Fos comparten propiedades similares de reconocimiento de ADN.

Conclusiones:

La proteína Fos influye directamente en la unión con el elemento regulador FSE2.
El sitio de unión TGACTCA identificado sugiere un papel directo de Fos en la transcripción mediada por AP-1.
Estos hallazgos indican una relación funcional entre Fos y el complejo del factor de transcripción AP-1.

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