Video Experimental Relacionado
Updated: Jan 21, 2026
09:57
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
Published on: February 4, 2016
11.2K
Fluoróforos BODIPY para la obtención de imágenes de potencial de membrana
Journal of the American Chemical Society
|July 25, 2019
Resumen
Los investigadores desarrollaron nuevos tintes BODIPY solubles en agua mediante la adición de un grupo aromático sulfonado. Estos tintes son útiles para crear fluoróforos sensibles al voltaje (VF) para imágenes biológicas en células y neuronas.
Área de la Ciencia:
- Química orgánica
- Biofísica y Química
- Ciencias de los materiales
Sus antecedentes:
- Los tintes BODIPY son valiosos fluoróforos debido a sus propiedades sintonizables y su compatibilidad biológica.
- Mejorar la solubilidad en agua de los tintes BODIPY es crucial para las aplicaciones biológicas, pero presenta desafíos sintéticos.
- Los métodos existentes para la solubilización en agua pueden interferir con las propiedades electrónicas necesarias para los indicadores funcionales.
Objetivo del estudio:
- Desarrollar una estrategia general para la creación de tintes BODIPY solubles en agua.
- Para sintetizar nuevos fluoroforos sensibles al voltaje (VF) basados en BODIPY para imágenes celulares.
- Evaluar el rendimiento de estos nuevos tintes en sistemas biológicos.
Principales métodos:
- Sintetizó cinco nuevos BODIPY con grupos aromáticos sulfonados en la posición meso.
- Funcionalizó estos BODIPY solubles en agua para crear trece nuevos fluoróforos sensibles a la tensión (VF) basados en BODIPY.
- Se probó la sensibilidad al voltaje y el brillo de los nuevos tintes VF en células y neuronas de mamíferos.
Principales resultados:
- Se ha demostrado que un grupo aromático sulfonado en la posición meso es una estrategia eficaz para los BODIPY solubles en agua.
- Desarrolló 13 nuevos fluoróforos sensibles a la tensión basados en BODIPY.
- Se ha logrado una sensibilidad al alto voltaje (48% ΔF/F por 100 mV) en las células de mamíferos con el colorante más sensible.
- Demostró la capacidad de estos tintes para informar sobre la dinámica del potencial de acción en las neuronas y los cardiomiocitos.
Conclusiones:
- La estrategia de incorporar un grupo aromático sulfonado proporciona una vía versátil para los fluoróforos de BODIPY solubles en agua.
- Los VF BODIPY desarrollados ofrecen una herramienta prometedora para estudiar la electrofisiología celular con un brillo y una sensibilidad mejorados.
- Este trabajo amplía el conjunto de herramientas para el diseño de indicadores fluorescentes funcionales con propiedades electrónicas sintonizables.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:21The Resting Membrane Potential
142.0K
Overview
142.0K
01:24Resting Membrane Potential
21.5K
The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
21.5K
00:52Potential Energy
42.3K
The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.3K
01:09Potential Energy
980
A conservative force, such as a gravitational or elastic force, gives the body the capacity to do work. This capacity, measured as the potential energy, depends on the body's location or “position” relative to a fixed reference position or datum. The gravitational potential energy is considered zero at the reference point. Suppose a body is located at some vertical distance above a fixed horizontal reference or datum. In that case, the weight of the body has positive gravitational potential...
980
03:02Standard Electrode Potentials
50.0K
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.0K
02:58Cell Potential and Free Energy
46.3K
Thermodynamics of a Redox Reaction
Thermodynamics is the branch of physics dealing with the relationship between heat and other forms of energy. In an electrochemical cell, chemical energy is converted into electrical energy.
Thus, a link can be predicted between cell potential, free energy change, and the equilibrium constant for the reaction. Cell potential can also be measured as the oxidant or the reducing strength, and similar acid-base strength measures are reflected in equilibrium...
Thermodynamics is the branch of physics dealing with the relationship between heat and other forms of energy. In an electrochemical cell, chemical energy is converted into electrical energy.
Thus, a link can be predicted between cell potential, free energy change, and the equilibrium constant for the reaction. Cell potential can also be measured as the oxidant or the reducing strength, and similar acid-base strength measures are reflected in equilibrium...
46.3K