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When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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La búsqueda de respuestas

Christian Jørgensen1, Katja Enberg2

  • 1Department of Biological Sciences, University of Bergen, Bergen, Norway. christian.jorgensen@uib.no.

Science (New York, N.Y.)
|August 3, 2019
PubMed
Resumen

No abstract available in PubMed .

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