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Biosíntesis de fosfonatos: aislamiento de la enzima responsable de la formación de un enlace carbono-fosforo
H M Seidel1, S Freeman, H Seto
1Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138.
Nature
|September 29, 1988
Resumen
Los investigadores identificaron la enzima fosfoenolpiruvato fosfonomutasa, que es crucial para crear el enlace fósforo-carbono en los fosfonatos. Este descubrimiento aclara el misterio de larga data de la biosíntesis de fosfonatos en organismos como Tetrahymena pyriformis.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Microbiología Microbiología.
Sus antecedentes:
- Los fosfonatos, que contienen un enlace fósforo-carbono, se encuentran en diversos organismos.
- La vía de biosíntesis para el enlace P-C en los fosfonatos ha permanecido en gran medida indefinida.
- Estudios anteriores sugirieron un papel para el fosfenol piruvato en la formación de fosfonatos, pero carecían de pruebas definitivas.
Objetivo del estudio:
- Para dilucidar la vía biosintética indefinida del enlace fósforo-carbono en los fosfonatos.
- Identificar y caracterizar la enzima responsable de la transformación del fosfenol piruvato en fosfonopiruvato.
Principales métodos:
- Experimentos de etiquetado de isótopos con el uso de [14C] glucosa y [32P] fosfenol piruvato en Tetrahymena pyriformis.
- Aislamiento y caracterización de enzimas de T. pyriformis.
- Investigando la transformación del fosfenol piruvato en fosfonopiruvato.
Principales resultados:
- El estudio informa de experimentos que resuelven el enigma de la biosíntesis de fosfonatos.
- Aislamiento y caracterización de la enzima pura, fosfoenolpiruvato fosfonomutasa, obtenida de T. pyriformis.
- Demostración del papel de la enzima en la transformación estequiométrica del fosfenol piruvato en fosfonopiruvato.
Conclusiones:
- Se confirma la existencia de la fosfonomutasa de fosfoenolpiruvato.
- Este descubrimiento aclara el misterio de larga data de la biosíntesis de fosfonatos.
- Los hallazgos proporcionan una base para comprender el significado biológico más amplio de los fosfonatos.
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