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Updated: May 9, 2026
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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
El proto-oncogén jun está autorregulado positivamente por su producto, Jun/AP-1
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, 92093.
Cell
|December 2, 1988
Resumen
El factor de transcripción Jun/AP-1 estimula directamente su propia expresión génica. Este bucle de retroalimentación positiva amplifica las señales en las células que responden a promotores tumorales como el TPA.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Reglamento genético Reglamento genético.
- Investigación de Investigación del Cáncer.
Sus antecedentes:
- El factor de transcripción Jun/AP-1 se une a las secuencias TRE, mejorando la transcripción génica celular en respuesta a estímulos como el TPA y los factores séricos.
- La actividad de Jun/AP-1 está elevada en las células estimuladas por TPA a través de la modificación post-traducional y el aumento de la expresión génica.
Objetivo del estudio:
- Investigar los mecanismos reguladores de la actividad de Jun/AP-1 en respuesta a la TPA.
- Aclarar el papel de Jun/AP-1 en la activación transcripcional de su propio gen.
Principales métodos:
- Mutagénesis específica del sitio del sitio de unión AP-1 en la región promotora juncional.
- Análisis de la inducción y transactivación de TPA por Jun/AP-1.
Principales resultados:
- La unión de Jun/AP-1 a un sitio de alta afinidad en el promotor de la unión media la transcripción inducida por TPA.
- La mutagenesis de este sitio de unión abolió la inducción de TPA y la trans-activación Jun/AP-1.
- Se demostró que la transcripción jun es estimulada directamente por su propio producto genético, Jun/AP-1.
Conclusiones:
- La autorregulación jun/AP-1 forma un bucle de retroalimentación positiva, estimulando directamente la transcripción del gen jun.
- Este bucle regulador positivo probablemente prolonga las señales celulares iniciadas por la activación de la proteína kinasa C.
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