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Unlike ethane and propane that have only two major conformations, butane has more than two conformers. The staggered form of butane in which the bulky methyl groups on the two carbons are placed on opposite sides, that is, at a dihedral angle of 180°, is the lowest energy, most stable form — called the anti conformer. This conformation is stabilized due to the absence of steric repulsion between the largely spaced out methyl groups. The other two staggered conformations are...
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Adolf von Baeyer attempted to explain the instabilities of small and large cycloalkane rings using the concept of angle strain — the strain caused by the deviation of bond angles from the ideal 109.5° tetrahedral value for sp3  hybridized carbons. However, while cyclopropane and cyclobutane are strained, as expected from their highly compressed bond angles, cyclopentane is more strained than predicted, and cyclohexane is virtually strain-free. Hence, Baeyer’s theory that...
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Cyclohexane does not exist in a planar form due to the high angle and torsional strain it would experience in the planar structure. Instead, it adopts non-planar chair and boat conformations.
The chair form is the most stable and derives its name from its resemblance to the “easy chair.” In the chair conformation, two carbon atoms are arranged out-of-plane — one above and one below, minimizing the torsional strain. In the chair form, the bond angle is very close to the ideal...
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Electricity is generated by either electrons or ions flowing through a solution or a conducting medium. This flow of electrons or specifically electrical charge is defined as an electric current. When electrons move through a wire, they generate an electric current. It can be recalled  that in a redox reaction, electrons are lost and gained. In the spontaneous redox reaction of zinc  with copper, when zinc is immersed in a copper ion solution, a transfer of electrons from one substance to...
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Spatial Separation of Molecular Conformers and Clusters
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Cambios de conformación inducidos por la fuerza en PIEZO1

Yi-Chih Lin1,2, Yusong R Guo3, Atsushi Miyagi1,2

  • 1Department of Anesthesiology, Weill Cornell Medicine, New York, NY, USA.

Nature
|August 23, 2019
PubMed
Resumen
Este resumen es generado por máquina.

El canal mecanicamente sensible PIEZO1, una proteína que convierte la fuerza en señales eléctricas, puede aplanarse reversiblemente en el plano de la membrana celular. Este cambio de forma es clave para cómo PIEZO1 responde a las fuerzas mecánicas.

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Área de la Ciencia:

  • La biofísica
  • Biología molecular
  • Biología celular

Sus antecedentes:

  • PIEZO1 es un canal iónico mecanicamente sensible crucial para la conversión de fuerzas mecánicas en señales celulares.
  • Estudios anteriores revelaron la estructura trímera en forma de cuenco de PIEZO1 con brazos extendidos.

Objetivo del estudio:

  • Investigar la dinámica estructural de PIEZO1 bajo diferentes condiciones mecánicas.
  • Aclarar el mecanismo por el cual PIEZO1 responde a la tensión y la fuerza de la membrana.

Principales métodos:

  • Se utilizó la crio-microscopía electrónica (cryo-EM) para observar la estructura de PIEZO1 en vesículas lipídicas de diferentes tamaños.
  • La microscopía de fuerza atómica de alta velocidad (HS-AFM) analizó la deformabilidad de PIEZO1 en membranas soportadas por mica.
  • Se aplicó fuerza mecánica para estimar la constante de resorte de PIEZO1.

Principales resultados:

  • PIEZO1 exhibe diversos grados de curvatura dependiendo del tamaño de la vesícula lipídica.
  • PIEZO1 puede aplanarse reversiblemente en el plano de la membrana cuando se somete a fuerza.
  • La constante de resorte mecánico de PIEZO1 se estimó utilizando mediciones de fuerza.

Conclusiones:

  • Se demuestra la capacidad de PIEZO1 para deformar su forma, particularmente aplanándose en una estructura plana.
  • Esta flexibilidad conformacional probablemente subyace a la conversión de la tensión lateral de la membrana en cambios de energía libre para la apertura del canal.
  • Los hallazgos proporcionan información sobre los mecanismos moleculares de la mecanotransducción por PIEZO1.