Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Induced-fit Model01:13

Induced-fit Model

88.9K
Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
88.9K
Goodness-of-Fit Test01:16

Goodness-of-Fit Test

8.1K
The goodness-of-fit test is a type of hypothesis test which determines whether the data "fits" a particular distribution. For example, one may suspect that some anonymous data may fit a binomial distribution. A chi-square test (meaning the distribution for the hypothesis test is chi-square) can be used to determine if there is a fit. The null and alternative hypotheses may be written in sentences or stated as equations or inequalities. The test statistic for a goodness-of-fit test is given as...
8.1K
Inclusive Fitness00:57

Inclusive Fitness

37.5K
Most altruistic behavior—in which one animal helps another at a cost to themselves—occurs between relatives. Scientists think these altruistic behaviors evolved because they increase the inclusive fitness of the animal providing help.
37.5K
Intermolecular Forces03:13

Intermolecular Forces

70.2K
Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
70.2K
Electromotive Force02:36

Electromotive Force

29.8K
Electricity is generated by either electrons or ions flowing through a solution or a conducting medium. This flow of electrons or specifically electrical charge is defined as an electric current. When electrons move through a wire, they generate an electric current. It can be recalled  that in a redox reaction, electrons are lost and gained. In the spontaneous redox reaction of zinc  with copper, when zinc is immersed in a copper ion solution, a transfer of electrons from one substance to...
29.8K
Expected Frequencies in Goodness-of-Fit Tests01:19

Expected Frequencies in Goodness-of-Fit Tests

7.2K
A goodness-of-fit test is conducted to determine whether the observed frequency values are statistically similar to the frequencies expected for the dataset. Suppose the expected frequencies for a dataset are equal such as when predicting the frequency of any number appearing when casting a die. In that case, the expected frequency is the ratio of the total number of observations (n)  to the number of categories (k).
7.2K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Metal-Phenolic Coatings Enable Universal Design of Spherical Nucleic Acids.

Angewandte Chemie (International ed. in English)·2026
Same author

Correction to "DNA-Mediated Cellular Delivery of Functional Enzymes".

Journal of the American Chemical Society·2026
Same author

High-χ Block Copolymer Nanoreactors for the Confined Synthesis of Size-Controlled Nanoclusters.

ACS nano·2026
Same author

Programmable Stepwise Heteroepitaxial Growth of Colloidal Crystals With Different Phases.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Simplex-based model for nanoparticle grain identification in four-dimensional scanning transmission electron microscopy data.

Journal of microscopy·2026
Same author

High-entropy 1D halide perovskite piezoelectrics found by megalibrary synthesis and rapid nonlinear optical screening.

Science advances·2026
Same journal

Radical Cascades on Seawater Microdroplets Drive Atmospheric Mercury Oxidation.

Journal of the American Chemical Society·2026
Same journal

Superior Selective and Fast NH<sub>3</sub> Adsorption of Soft Porous MOF/Ionic Liquid Composites with Ordering Phase Transitions.

Journal of the American Chemical Society·2026
Same journal

Systematic Catalyst Variation for Improved Stereoselective Epoxide Polymerization: Subtle Modifications Resulting in Superior Efficiency.

Journal of the American Chemical Society·2026
Same journal

Deciphering the Halide Chemistry of Cl<sup>-</sup> and Br<sup>-</sup> in Enhancing Kinetics of Mg Plating/Stripping.

Journal of the American Chemical Society·2026
Same journal

Electrosynthesis of C<sub>6</sub> Chemicals by Propylene Oxidative Coupling on Au Surface.

Journal of the American Chemical Society·2026
Same journal

Statistical AI Enables Precise Screening of Multielement Catalysts.

Journal of the American Chemical Society·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Jan 20, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.2K

Intercalación forzada (FIT) - Aptamers

Sasha B Ebrahimi, Devleena Samanta, Ho Fung Cheng

    Journal of the American Chemical Society
    |August 24, 2019
    PubMed
    Resumen
    Este resumen es generado por máquina.

    Los investigadores desarrollaron aptameros de intercalación forzada (FIT), una nueva clase de aptameros de señalización. Estos aptámeres utilizan una sola modificación para permitir la detección de fluorescencia sensible y específica de varios objetivos en muestras biológicas complejas.

    Más Videos Relacionados

    Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
    08:23

    Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

    Published on: June 23, 2016

    12.7K
    Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
    08:11

    Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

    Published on: August 9, 2022

    2.2K

    Videos de Experimentos Relacionados

    Last Updated: Jan 20, 2026

    Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
    08:09

    Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

    Published on: January 7, 2017

    11.2K
    Design and Development of Aptamer&#8211;Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
    08:23

    Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

    Published on: June 23, 2016

    12.7K
    Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
    08:11

    Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

    Published on: August 9, 2022

    2.2K

    Área de la Ciencia:

    • La bioquímica
    • Biología molecular
    • Química analítica

    Sus antecedentes:

    • Los aptameros son herramientas de oligonucleótidos versátiles para la detección del analito.
    • Los métodos de señalización de aptamer existentes a menudo sufren de falsos positivos o un rango dinámico limitado.

    Objetivo del estudio:

    • Desarrollar una estrategia general de diseño de aptamer para una lectura de fluorescencia robusta.
    • Crear una nueva clase de aptámeres de señalización con características de rendimiento mejoradas.

    Principales métodos:

    • Utilizó un tinte sensible a la viscosidad que se intercala en complejos de aptamer-objetivo.
    • Desarrolló aptameros de intercalación forzada (FIT) que aumentan la fluorescencia al unirse al objetivo.
    • Utilidad demostrada del aptamer FIT en varios modos de unión, incluidos los cambios conformacionales, los ensayos sandwich y la reassociación del aptamer split.

    Principales resultados:

    • Los aptámeros FIT lograron un aumento de hasta 20 veces en la fluorescencia tras la unión al objetivo.
    • La estrategia detectó con éxito la formación de pares metalo-base.
    • En comparación con los sistemas tradicionales, los aptámeres FIT mostraron una reducción de las señales de falsos positivos.
    • Se han logrado altas proporciones de señal a fondo (hasta 15 veces más altas que FRET) y sensibilidad nanomolar en suero humano.

    Conclusiones:

    • Los aptámeros FIT representan una nueva clase de aptámeros de señalización con una sola modificación.
    • Esta estrategia permite la detección sensible y específica de diversos objetivos en medios complejos.
    • Los aptameros FIT ofrecen una alternativa prometedora a los métodos de detección basados en aptameros existentes.