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Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

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The protocol presents a series of best practice protocols for the collection of bone powder from eight recommended anatomical sampling locations (specific locations on a given skeletal element) across five different skeletal elements from medieval individuals (radiocarbon dated to a period of ca. 1040-1400 CE, calibrated 2-sigma...
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Rescue of recombinant arenaviruses from cloned cDNAs, an approach referred to as reverse genetics, allows researchers to investigate the role of specific viral gene products, as well as the contribution of their different specific domains and residues, to many different aspects of the biology of arenavirus. Likewise, reverse genetics techniques in FDA-approved cell lines (Vero) for vaccine development provides novel possibilities for the generation of effective and safe vaccines to combat human...
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This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called...
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Stable carbon and oxygen isotope analysis of human and animal tooth enamel carbonate has been used as a proxy for individual diet and environmental reconstruction. Here, we provide a detailed description and visual documentation of bulk and sequential tooth enamel sampling as well as pretreatment of archaeological and paleontological...
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Minimum Volume Vitrification of Immature Feline Oocytes07:16

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This manuscript describes a protocol for the minimum volume vitrification of immature cat oocytes with laboratory-made media on commercial supports. It covers every step from oocyte isolation from ex vivo gonads to vitrification and...
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Video Experimental Relacionado

Updated: Jan 20, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

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Arqueología galáctica con Gaia

Rosemary Wyse1

  • 1Department of Physics and Astronomy, Johns Hopkins University, Baltimore, MD, USA. wyse@jhu.edu.

Science (New York, N.Y.)
|September 7, 2019
PubMed
Resumen

No abstract available in PubMed .

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