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Redes dinámicas constitucionales tridimensionales basadas en ácido nucleico: mejora de la diversidad a través de la
Liang Yue1, Shan Wang1, Itamar Willner1
1Institute of Chemistry, The Center for Nanoscience and Nanotechnology , The Hebrew University of Jerusalem , Jerusalem 91904 , Israel.
Journal of the American Chemical Society
|September 21, 2019
Resumen
Los científicos crearon redes dinámicas constitucionales tridimensionales (3D CDNs) utilizando ácidos nucleicos. Estas redes adaptables se pueden reconfigurar y controlar con precisión utilizando disparadores, lo que demuestra nuevas capacidades de ensamblaje molecular.
Área de la Ciencia:
- Química supramolecular
- Biología sintética
- Nanotecnología
Sus antecedentes:
- La naturaleza utiliza redes reguladoras de genes con constituyentes multicomponentes para el desarrollo biológico.
- Las secuencias de bases de ácido nucleico codifican información para la construcción molecular.
Objetivo del estudio:
- Construir redes dinámicas constitucionales tridimensionales (3D CDNs) a partir de ácidos nucleicos.
- Demostrar la reconfiguración adaptativa y el control jerárquico sobre la composición de la CDN utilizando disparadores.
- Desarrollar métodos cuantitativos para evaluar el contenido de los componentes de la CDN.
Principales métodos:
- Construcción de CDN 3D utilizando ocho componentes de ácido nucleico de tres componentes (ABC).
- Aplicación de cuatro disparadores de ácido nucleico auxiliares (T1-T4) para la reconfiguración de la red.
- Etiquetado de los componentes con reporteros de ADNenzimas dependientes de iones Mg2+ y un complejo de cocaína-aptámero para su cuantificación.
- Utilizando la actividad de la enzima del ADN y las señales de fluorescencia para el análisis cuantitativo, apoyado por la electroforesis.
Principales resultados:
- Reconfiguración adaptativa de una CDN 3D madre (CDN I) en cuatro CDN distintas (II-V) mediante el uso de disparadores individuales.
- Demostrar el control jerárquico reversible de los nuevos sistemas CDN (VI-IX) mediante disparadores consecutivos o contrarios.
- Evaluación cuantitativa exitosa del contenido de los constituyentes en varias CDN utilizando reporteros de ADNzima y ensayos de fluorescencia.
Conclusiones:
- Las CDN 3D basadas en ácido nucleico exhiben una reconfiguración adaptativa y controlable.
- Las estrategias de etiquetado y cuantificación desarrolladas permiten una evaluación precisa de la composición de la CDN.
- Este trabajo proporciona una base para sistemas moleculares sofisticados y programables.
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