Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
RNA Splicing01:32

RNA Splicing

60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K
RNA-seq03:21

RNA-seq

11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.7K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.2K
5.2K
Overview of Exosomes01:36

Overview of Exosomes

3.6K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.6K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

The E3-ome gene-centric compendium reveals the human E3 ligase landscape.

Cell·2026
Same author

Structural basis for a nucleoporin exportin complex between RanBP2, SUMO1-RanGAP1, the E2 Ubc9, Crm1 and the Ran GTPase.

Nature communications·2025
Same author

Structural basis for a nucleoporin exportin complex between RanBP2, SUMO1-RanGAP1, the E2 Ubc9, Crm1 and the Ran GTPase.

bioRxiv : the preprint server for biology·2025
Same author

Chemical Tools for Probing the Ub/Ubl Conjugation Cascades.

Chembiochem : a European journal of chemical biology·2024
Same author

Structural basis for transthiolation intermediates in the ubiquitin pathway.

Nature·2024
Same author

Design and Semisynthesis of Biselectrophile-Functionalized Ubiquitin Probes To Investigate Transthioesterification Reactions.

Organic letters·2024

Video Experimental Relacionado

Updated: Jan 19, 2026

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

100.4K

SnapShot: El exosoma del ARN

M Rhyan Puno1, Eva-Maria Weick1, Mom Das1

  • 1Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10065.

Cell
|September 21, 2019
PubMed
Resumen

El exosoma de ARN es una máquina celular vital que degrada el ARN. Esta descripción detalla su estructura, función y proteínas asociadas en las células eucariotas.

Más Videos Relacionados

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

8.6K
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

11.6K

Videos de Experimentos Relacionados

Last Updated: Jan 19, 2026

Isolation and Characterization of RNA-Containing Exosomes
09:43

Isolation and Characterization of RNA-Containing Exosomes

Published on: January 9, 2012

100.4K
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

8.6K
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

11.6K

Área de la Ciencia:

  • Biología molecular
  • La bioquímica
  • Biología celular

Sus antecedentes:

  • El exosoma de ARN es un complejo de ribonucleasa de 3' a 5' crucial.
  • Es esencial para el metabolismo del ARN, incluida la maduración, el control de calidad y la degradación.
  • Este complejo se conserva en los organismos eucariotas.

Objetivo del estudio:

  • Para proporcionar una visión general del exosoma de ARN.
  • Describir su estructura, composición y diversas funciones.
  • Resaltar las funciones de los cofactores asociados y los complejos proteicos.

Principales métodos:

  • Revisión de la literatura y síntesis de las investigaciones existentes.
  • Análisis de los datos estructurales y funcionales del exosoma de ARN.
  • Compilación de información sobre los componentes de los exosomas nucleares y del citoplasma.

Principales resultados:

  • Descripción detallada de la estructura central y las subunidades del exosoma de ARN.
  • Elucidación de sus funciones en el procesamiento de varios tipos de ARN (ARNm, rRNA, snRNA, etc.) En el caso de los
  • Identificación y caracterización de los cofactores exosómicos clave y de los complejos asociados.

Conclusiones:

  • El exosoma de ARN es un complejo de múltiples subunidades con funciones indispensables en la homeostasis del ARN eucariota.
  • Sus funciones están moduladas por una red de cofactores y proteínas accesorias.
  • Comprender el exosoma de ARN es crítico para comprender la expresión génica y la regulación celular.