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Positive Regulator Molecules01:45

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To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
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The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
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Positive Regulator Molecules02:39

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Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
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M-Cdk Drives Transition Into Mitosis02:15

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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
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Separation of Sister Chromatids02:17

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Anaphase Promoting Complex00:50

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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Sincronización del compromiso de las células MEL con la cordycepsina.

R Levenson, J Kernen, D Housman

    Cell
    |December 1, 1979
    PubMed
    Resumen

    La cordicépina, un análogo de nucleótido, bloquea la diferenciación de los eritroides en las células de la eritroleucemia (MEL) del ratón. Este hallazgo revela un nuevo mecanismo molecular que controla el compromiso celular con la diferenciación terminal.

    Área de la Ciencia:

    • * Biología molecular.
    • * Diferenciación celular de las células.
    • * Investigación sobre el cáncer.

    Sus antecedentes:

    • * Las células de eritroleucemia de ratón (MEL) son un sistema modelo para el estudio de la diferenciación eritroide.
    • * El compromiso con la diferenciación terminal implica eventos moleculares complejos.
    • * Los análogos de nucleótidos pueden modular los procesos celulares.

    Objetivo del estudio:

    • * Para investigar el efecto de la cordicépina en la diferenciación celular de MEL.
    • * Para identificar el papel de la cordicépina en los eventos moleculares del compromiso eritreo.
    • * Explorar el potencial de la cordicépina para bloquear la diferenciación en una etapa específica.

    Principales métodos:

    • * Tratamiento de las células MEL diferenciadas con dosis variables de cordicépina.

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  • * Evaluación del compromiso celular y la citotoxicidad.
  • * Experimentos de reversión con cordicépina en presencia de un inductor.

    • Principales resultados:

    • * La cordicépina inhibe rápidamente el compromiso de las células MEL con la diferenciación eritroide.
    • * La inhibición se produce en dosis no citotóxicas.
    • * La reversión del tratamiento con cordicépina permite un compromiso celular rápido y sincrónico.

    Conclusiones:

    • * Cordycepin descubre un aspecto no reconocido previamente del compromiso de diferenciación eritroide.
    • * Las células MEL pueden ser detenidas justo antes del compromiso por cordycepin.
    • * Cordycepin ofrece una herramienta para estudiar el tiempo molecular preciso de las decisiones del destino celular.