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La mecanización de las uniones estrechas depende de la separación y el flujo de la fase ZO-1
Cornelia Schwayer1, Shayan Shamipour1, Kornelija Pranjic-Ferscha1
1Institute of Science and Technology Austria, Klosterneuburg, Austria.
Cell
|November 2, 2019
Resumen
Las uniones célula-célula adquieren sensibilidad mecánica a través de la formación y el flujo de Zonula Occludens-1. Este proceso, impulsado por la tensión de la actomiosina, es crucial para el desarrollo adecuado de los tejidos y el movimiento de la capa celular.
Área de la Ciencia:
- Biología celular
- La biofísica
- Biología del desarrollo
Sus antecedentes:
- Las uniones célula-célula, particularmente las uniones cerradas (TJ), responden dinámicamente a las fuerzas mecánicas.
- La comprensión de la base mecanoquímica de la mecaniosensibilidad de las uniones es crucial para comprender la morfogénesis tisular.
Objetivo del estudio:
- Investigar los mecanismos mecanoquímicos subyacentes a la mecanicidad de las uniones cerradas (TJ).
- Para analizar el papel de Zonula Occludens-1 (ZO-1) en la respuesta de TJ a las fuerzas mecánicas durante la gastrulación del pez cebra.
Principales métodos:
- Análisis de la formación de TJ entre la capa celular envolvente (EVL) y la capa sincitial de la yema (YSL) en embriones de pez cebra.
- Cuantificación de la acumulación de ZO-1 en TJ y su correlación con la tensión de la red de actomiosina.
- Investigación de la formación de cúmulos ZO-1 a través de la separación de fases y su incorporación en TJ.
Principales resultados:
- La acumulación de ZO-1 en las escalas de TJ con tensión de actomiosina, lo que indica la mecanicidad de TJ.
- La tensión de la actomyosina impulsa el flujo retrógrado de los grupos ZO-1 separados por fase hacia los TJ.
- La formación o incorporación alterada de ZO-1 elimina la mecanosensibilidad TJ y retrasa el movimiento EVL-YSL.
Conclusiones:
- La separación de fase y el flujo retrógrado de la ZO-1 no juncional confieren mecanicidad a las TJ.
- La unión a la actina de ZO-1 es esencial para su incorporación estable a las TJ.
- Los TJs mecano-sensibles son críticos para el movimiento coordinado de la capa celular durante el desarrollo embrionario.
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