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Un antiguo provirus ha impuesto la regulación de andrógenos en el gen de la proteína limitada por el sexo del ratón
1Department of Biological Sciences, Columbia University, New York, New York 10027.
Cell
|October 21, 1988
Resumen
Una antigua inserción de provirus cerca del gen de la proteína limitada por el sexo (Slp) del ratón confería la regulación de los andrógenos. Esta mutación de inserción a largo plazo demuestra el mantenimiento evolutivo de un elemento transponible que influye en la expresión génica.
Área de la Ciencia:
- Genética La genética.
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- La expresión del gen de la proteína limitada por el sexo (Slp) del ratón es dependiente de los andrógenos, y difiere de su vecino, el gen del componente 4 (C4) del complemento.
- La identidad de alta secuencia entre Slp y C4 sugiere un origen evolutivo compartido.
Objetivo del estudio:
- Investigar el mecanismo regulador detrás de la expresión dependiente de andrógenos del gen Slp.
- Identificar el elemento genético responsable de conferir la respuesta hormonal al gen Slp.
Principales métodos:
- Análisis comparativo de la secuencia de los genes Slp y C4.
- Identificación y caracterización de los elementos genéticos insertados aguas arriba del gen Slp.
- Análisis de secuencias y mutaciones de repetición terminal larga (RTL) retrovirales.
Principales resultados:
- Se identificó un provirus endógeno aproximadamente 2 kb aguas arriba del gen Slp.
- El 5' LTR de este provirus contiene un elemento potenciador de respuesta hormonal.
- La divergencia de secuencias y las mutaciones indican que el provirus es antiguo y está integrado de manera estable en el genoma del ratón.
Conclusiones:
- La antigua inserción de provirus ha conferido una respuesta androgénica al gen Slp adyacente.
- Esto representa un raro ejemplo de una mutación de inserción de un elemento transponible que se mantiene evolutivamente.
- Los hallazgos proporcionan información sobre el impacto a largo plazo de los retrovirus endógenos en la regulación y evolución del genoma del huésped.
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