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Interacciones moleculares del receptor de la hormona esteroide con su elemento potenciador: evidencia para la

S Y Tsai¹, J Carlstedt-Duke, N L Weigel

¹Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.

|October 21, 1988

Resumen

Los receptores de hormonas esteroideas, incluidos los receptores de glucocorticoides y progesterona, se unen directamente a los elementos de respuesta del ADN. Estos receptores interactúan con el ADN como dímeros, con sitios de unión específicos identificados para la unión cooperativa.

Área de la Ciencia:

Biología molecular La biología molecular.
Endocrinología Endocrinología.
Genética La genética.

Sus antecedentes:

Los receptores de hormonas esteroideas regulan la expresión génica al unirse a secuencias específicas de ADN.
Los receptores de glucocorticoides y progesterona (GR/PR) reconocen elementos de respuesta similares (GRE/PRE).

Objetivo del estudio:

Para investigar las interacciones moleculares entre los receptores de la hormona esteroide y sus elementos de respuesta del ADN.
Para identificar sitios específicos de unión y patrones de dimerización de GR/PR en GRE/PRE.

Principales métodos:

Se emplearon ensayos de desplazamiento de banda para estudiar las interacciones receptor-ADN.
Se utilizaron fragmentos de péptidos bacterianos del dominio de unión al ADN del receptor de glucocorticoides.

Principales resultados:

Tanto los receptores de progesterona como los de glucocorticoides se unen específicamente al GRE/PRE.
Se observaron sitios de contacto con purinas idénticos para el receptor de progesterona de pollo (formas A y B) y el receptor de glucocorticoides de rata.
Se demostró la unión cooperativa de los dímeros de los receptores a los semisitios GRE/PRE (TGTTCT y TGTACA).

Conclusiones:

Los receptores de la hormona esteroide interactúan con sus potenciadores cognados como dimeros.
El dominio de unión al ADN media la unión específica y cooperativa al GRE/PRE.

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