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Transferencia de nitreno catalizada por una enzima de hierro no hemo y mejorada por ligandos de moléculas pequeñas no

Nathaniel W Goldberg, Anders M Knight, Ruijie K Zhang

Journal of the American Chemical Society

|December 3, 2019

Resumen

La enzima formadora de etileno de Pseudomonas savastanoi, una enzima de hierro no hemo, cataliza la aziridinación de la olefina y la inserción de C-H. La evolución dirigida mejoró estas actividades catalíticas, con pequeñas moléculas que controlan la selectividad de las enzimas.

Área de la Ciencia:

Biocatálisis y química sintética
Ingeniería enzimática y evolución dirigida
Química organometálica y catálisis

Sus antecedentes:

La catálisis de metales de transición es crucial para la formación de enlaces químicos.
Las enzimas de hierro no hemo ofrecen propiedades catalíticas únicas.
La enzima formadora de etileno de Pseudomonas savastanoi (EFE) es una enzima de hierro no hemo.

Objetivo del estudio:

Investigar las capacidades catalíticas de Pseudomonas savastanoi EFE más allá de su función nativa.
Explorar el potencial de la evolución dirigida para mejorar las actividades catalíticas de la EFE.
Demostrar el uso de moléculas pequeñas para controlar la selectividad de las enzimas.

Principales métodos:

Utilizando el Pseudomonas savastanoi EFE como biocatalizador.
Emplear técnicas de evolución dirigida para mejorar el rendimiento de las enzimas.
Investigando las reacciones de inserción de aziridinación de olefinas y nitreno C-H.
Modificando el sitio activo de la enzima con moléculas de coordinación de metales.

Principales resultados:

Se ha demostrado que el Pseudomonas savastanoi EFE puede catalizar la aziridinación de la olefina.
Demostró la capacidad de la enzima para realizar reacciones de inserción de nitreno C-H.
Se logró una mayor actividad catalítica y selectividad a través de la evolución dirigida.
Se estableció que las pequeñas moléculas pueden modular la actividad y la selectividad de las enzimas a través de la modificación de la esfera de coordinación.

Conclusiones:

Pseudomonas savastanoi EFE es un biocatalizador versátil para transformaciones químicas difíciles.
La evolución dirigida es una estrategia efectiva para optimizar la función de las enzimas.
El centro de hierro no hemo de EFE proporciona una plataforma para la catálisis sintonizable utilizando moléculas pequeñas.

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