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Multiplexado temporal en la conversión de imágenes en vivo

Hui Li1, Meiling Tan1, Xin Wang1

  • 1MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering & Key Laboratory of Micro-systems and Micro-structures, Ministry of Education , Harbin Institute of Technology , Harbin 150001 , People's Republic of China.

Journal of the American Chemical Society
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PubMed
Resumen
Este resumen es generado por máquina.

Las nuevas nanopartículas de conversión ascendente (UCNPs) permiten imágenes multiplexadas in vivo mediante el uso de vidas de luminiscencia distintas. Este avance permite imágenes multicolores de alto contraste en las profundidades de los tejidos, avanzando la bioimagen y el diagnóstico.

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Área de la Ciencia:

  • Nanotecnología
  • Ingeniería biomédica
  • Ciencias de los materiales

Sus antecedentes:

  • Las nanopartículas de conversión ascendente (UCNPs) convierten la luz del infrarrojo cercano (NIR) en luminiscencia visible, crucial para la bioimagen.
  • Las imágenes multiplexadas in vivo están limitadas por la escasez de bandas de luminiscencia UCNP que penetran en los tejidos profundos.

Objetivo del estudio:

  • Desarrollar un nuevo enfoque para la conversión múltiple en imágenes in vivo utilizando la discriminación del dominio temporal.
  • Para superar las limitaciones de las bandas de luminiscencia UCNP existentes para la penetración en tejidos profundos.

Principales métodos:

  • Nanoestructuras de tetradominio diseñadas para controlar la migración de energía y los procesos de conversión ascendente.
  • Discriminación del dominio de tiempo de la luminiscencia NIR (808 nm) con tiempos de vida distintos.
  • Se administraron UCNP acuosos por vía intravenosa y subcutánea a ratones de Kunming.

Principales resultados:

  • Luminescencia de conversión de alto rendimiento cuántico (máximo ≈ 6,1%) con vidas controladas con precisión que abarcan dos órdenes de magnitud (78 2157 μs).
  • Se ha demostrado la obtención de imágenes de alto contraste y color durante toda la vida de UCNPs en el hígado y los tejidos subcutáneos de ratones.
  • Presentación multicolor habilitada de imágenes descifradas utilizando patrones ópticos y vidas definidas.

Conclusiones:

  • El enfoque de la conversión temporal múltiple permite la obtención de imágenes avanzadas in vivo y la lucha contra la falsificación.
  • Este método tiene implicaciones significativas para el biosensing de alto rendimiento, las pantallas volumétricas y el diagnóstico y la terapia.