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Published on: September 21, 2011
Múltiples y cooperativos dominios de transactivación de los receptores de glucocorticoides humanos
1Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, California.
Cell
|December 2, 1988
Resumen
Los investigadores identificaron dos dominios de activación dentro del receptor de glucocorticoides humano (hGR). Estas secuencias, tau 2 y otra en el extremo amino, son cruciales para la función del receptor y la inducibilidad hormonal.
Área de la Ciencia:
- Biología molecular La biología molecular.
- Endocrinología Endocrinología.
- Genética La genética.
Sus antecedentes:
- El receptor glucocorticoide humano (hGR) es un receptor nuclear clave que regula la expresión génica.
- Los dominios de activación son críticos para la actividad transcripcional de los receptores nucleares.
Objetivo del estudio:
- Para identificar y caracterizar los dominios de activación funcional dentro de la hGR.
- Para investigar las propiedades y la localización de estos dominios de activación.
Principales métodos:
- Cartografía de péptidos y ensayos funcionales utilizando proteínas de fusión GAL4 de levadura.
- Análisis de las características de la secuencia y la modularidad funcional.
Principales resultados:
- Un péptido de 30 aminoácidos (tau 2) en el extremo carboxilo de la hGR funciona como un potente dominio de activación.
- Tau 2 confiere actividad transcripcional inducible por hormonas cuando se fusiona con GAL4.
- Se identificó un segundo dominio de activación independiente en el amino terminus de hGR.
- Ambos dominios son ácidos y funcionan independientemente de su posición.
Conclusiones:
- El hGR contiene al menos dos dominios de activación distintos, uno en el extremo carboxilo (tau 2) y otro en el extremo amino.
- Estos dominios son esenciales para el papel de la hGR como factor de transcripción inducible por hormonas.
- La naturaleza ácida de estos dominios puede ser una característica común compartida con las secuencias de activadores de levadura.
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