Video Experimental Relacionado

Updated: Dec 29, 2025

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

Caracterización genómica y epidemiología del nuevo coronavirus 2019: implicaciones para los orígenes del virus y la

Roujian Lu¹, Xiang Zhao¹, Juan Li²

¹NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Lancet (London, England)

|February 3, 2020

Resumen

Se identificó un nuevo coronavirus (2019-nCoV) que causa neumonía viral en Wuhan, China. El análisis genético lo revela.

Área de la Ciencia:

Virología
La genómica
Epidemiología

Sus antecedentes:

Identificado por primera vez en Wuhan, China, a fines de diciembre de 2019, un nuevo coronavirus (2019-nCoV) causó neumonía viral de origen desconocido.
Se confirmó la transmisión de persona a persona, con más de 2000 casos reportados hasta el 26 de enero de 2020, afectando principalmente a personas en Wuhan.

Objetivo del estudio:

Secuenciar y analizar los genomas del nuevo coronavirus (2019-nCoV) de los pacientes infectados.
Determinar los orígenes evolutivos y las propiedades de unión al receptor del 2019-nCoV.

Principales métodos:

La secuenciación de próxima generación y la secuenciación de Sanger se utilizaron para obtener genomas completos y parciales de 2019-nCoV de nueve pacientes.
El análisis filogenético y el modelado de homología se emplearon para comprender la historia evolutiva del virus y las posibles interacciones con los receptores.

Principales resultados:

Se obtuvieron diez secuencias del genoma del 2019-nCoV muy similares (>99.98% de identidad).
El 2019-nCoV mostró una identidad del 88% con los coronavirus similares al SARS derivados de murciélagos, distintos del SARS-CoV y el MERS-CoV.
El modelado de homología indicó una estructura de dominio de unión al receptor similar al SARS-CoV, lo que sugiere una posible unión a los receptores ACE2 humanos.

Conclusiones:

El 2019-nCoV es un betacoronavirus distinto que infecta a los humanos, que es diferente del SARS-CoV.
Los murciélagos son el probable huésped original, con un potencial huésped animal intermedio en el mercado de mariscos de Wuhan.
Se requiere una investigación urgente sobre la evolución futura, la adaptación y la propagación del virus.

Más Videos Relacionados

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

Published on: November 10, 2023

Videos de Conceptos Relacionados

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

G Protein-coupled Receptors

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...