Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Sanger Sequencing01:57

Sanger Sequencing

772.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
772.3K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.4K
Next-generation Sequencing03:00

Next-generation Sequencing

97.3K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.3K
RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Reply to: "Hypoxia-Induced Lineage Plasticity in Neuroblastoma: Advancing Signature Interpretation and Clinical Translation".

Clinical and translational science·2026
Same author

Maternal trans-vaccenic acid shapes neonatal T cell development and early-life immune imprinting.

Science (New York, N.Y.)·2026
Same author

Spatially resolved m<sup>6</sup>A profiling using m<sup>6</sup>A-ARTR-DBiT.

Nature methods·2026
Same author

CDS-localized m<sup>6</sup>A drives co-translational RNA decay to relieve biotic and abiotic endoplasmic reticulum stresses.

Nature plants·2026
Same author

Regulatory elements on chromatin-associated RNA 15 years beyond RNA epigenetics.

Nature chemical biology·2026
Same author

mRNA m<sup>6</sup>A modifications and the RNA-binding protein YTHDF1 affect translational control in both normal and pathological learning.

Proceedings of the National Academy of Sciences of the United States of America·2026

Video Experimental Relacionado

Updated: Dec 28, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.9K

Amplificación y secuenciación específica del ADN 5-metilcitosina

Chang Liu1,2, Xiaolong Cui1,2, Boxuan Simen Zhao3

  • 1Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois 60637, United States.

Journal of the American Chemical Society
|February 21, 2020
PubMed
Resumen

Este estudio introduce un nuevo método de amplificación de todo el genoma para el mapeo preciso de la 5-metilcitosina (5mC) del ADN. La nueva técnica garantiza la retención de 5mC a partir de un ADN mínimo, superando las limitaciones de los métodos anteriores.

Más Videos Relacionados

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
06:26

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Published on: October 5, 2012

12.3K
High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

15.3K

Videos de Experimentos Relacionados

Last Updated: Dec 28, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.9K
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
06:26

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Published on: October 5, 2012

12.3K
High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

15.3K

Área de la Ciencia:

  • La epigenética
  • Biología molecular
  • La genómica

Sus antecedentes:

  • La secuenciación convencional de bisulfito para el mapeo de la 5-metilcitosina (5mC) sufre de degradación del ADN.
  • La presencia de 5-hidroximetilcitosina (5hmC) complica la detección precisa de 5mC mediante el uso de métodos estándar.

Objetivo del estudio:

  • Desarrollar un método de amplificación del ADN que mapee específicamente la 5-metilcitosina (5mC) con alta precisión.
  • Para superar las limitaciones de la degradación del ADN y la interferencia 5hmC en el análisis del metiloma 5mC.

Principales métodos:

  • Se desarrolló una nueva técnica de amplificación de todo el genoma específica de 5mC (5mC-WGA).
  • El método fue optimizado para una entrada de ADN tan baja como 10 pg.
  • Se evaluó la retención de 5mC y la interferencia de las señales de 5hmC.

Principales resultados:

  • El método 5mC-WGA retuvo con éxito las señales de 5mC durante la amplificación del ADN.
  • Se observó una interferencia mínima de las señales de 5-hidroximetilcitosina (5hmC).
  • Se logró una alta reproducibilidad y precisión en el perfil del metiloma de ADN 5mC.

Conclusiones:

  • El método 5mC-WGA desarrollado proporciona una solución robusta para el análisis preciso del ADN 5mC metiloma.
  • Esta técnica permite estudios epigenéticos confiables incluso con una entrada limitada de ADN.
  • Mejora significativamente la precisión del mapeo de 5mC en comparación con los enfoques convencionales.