Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

DNA Topoisomerases02:02

DNA Topoisomerases

34.4K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
34.4K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.3K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.3K
Condensins02:15

Condensins

4.4K
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
4.4K
DNA Helicases00:55

DNA Helicases

23.6K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
23.6K
The DNA Replication Fork01:02

The DNA Replication Fork

40.1K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
40.1K
The DNA Replication Fork01:02

The DNA Replication Fork

17.8K
17.8K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Devising a divisome for synthetic cells.

Nature reviews. Chemistry·2026
Same author

Terminal Conjugation Enables Nanopore Sequencing of Peptides.

Journal of the American Chemical Society·2026
Same author

Two CTCF motifs impede cohesin-mediated DNA loop extrusion.

Molecular cell·2025
Same author

Telomeres stall DNA loop extrusion by condensin.

Cell reports·2025
Same author

Cohesin supercoils DNA during loop extrusion.

Cell reports·2025
Same author

The fine art of chromatin folding: Revealing the path of DNA inside mitotic chromosomes.

Cell genomics·2025

Video Experimental Relacionado

Updated: Dec 27, 2025

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

7.0K

Los complejos de condensina de extrusión de bucle de ADN pueden atravesarse entre sí

Eugene Kim1, Jacob Kerssemakers1, Indra A Shaltiel2

  • 1Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, The Netherlands.

Nature
|March 6, 2020
PubMed
Resumen

Dos complejos de proteínas de condensina de levadura (SMC) interactúan para empacar el ADN. Forman nuevas estructuras de bucle Z, lo que permite la compactación dinámica del ADN crucial para la organización cromosómica.

Más Videos Relacionados

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.8K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.3K

Videos de Experimentos Relacionados

Last Updated: Dec 27, 2025

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

7.0K
Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

15.8K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

1.3K

Área de la Ciencia:

  • Biología molecular
  • La bioquímica
  • La genética

Sus antecedentes:

  • La condensina, un complejo de mantenimiento estructural del cromosoma (SMC), actúa como un motor del ADN.
  • La acción colectiva de la condensina en el empaque del ADN sigue siendo poco conocida.

Objetivo del estudio:

  • Investigar las interacciones entre dos complejos de condensina de levadura que extruyen el bucle de ADN.
  • Para aclarar los mecanismos de envasado cooperativo del ADN por la condensa.

Principales métodos:

  • Técnicas de visualización de una sola molécula en lapso de tiempo.
  • Observación de las interacciones mutuas entre los motores individuales de condensina de levadura.

Principales resultados:

  • Los complejos de condensina alteran dinámicamente el tamaño del bucle de ADN de cada uno, independientemente de la distancia.
  • La proximidad permite que los complejos de condensina formen bucles Z, una estructura con tres hélices de ADN paralelas.
  • Los bucles Z facilitan el llenado de huecos y crean motores de dímeros simétricos para la extracción de ADN.

Conclusiones:

  • La condensina utiliza diversas estructuras de bucle, incluidos los bucles Z, para una compactación cromosómica eficiente.
  • Las interacciones entre los motores de condensación son clave para el empaquetado colectivo del ADN y la organización cromosómica.