Video Experimental Relacionado

Updated: Dec 26, 2025

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

La regulación del grupo de RNAPII es integral para la respuesta al daño del ADN

Ana Tufegdžić Vidaković¹, Richard Mitter², Gavin P Kelly²

¹Mechanisms of Transcription Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

|March 7, 2020

Resumen

La ubiquitación de la ARN polimerasa II (RNAPII) en RPB1 K1268 coordina las respuestas al daño del ADN, incluida la reparación y la degradación de RNAPII. Esta regulación es crucial para la supervivencia celular y la recuperación de la transcripción.

Área de la Ciencia:

Biología molecular
Biología celular
La genética

Sus antecedentes:

Las células activan respuestas complejas al daño del ADN, incluida la reparación del ADN y el cierre de la transcripción.
La degradación de la ARN polimerasa II (RNAPII) es un evento clave en la respuesta al daño del ADN.

Objetivo del estudio:

Para investigar el mecanismo de coordinación que vincula las respuestas al daño del ADN.
Para aclarar el papel de la ubicuidad del RNAPII en la respuesta al daño del ADN.

Principales métodos:

Enfoque en la ubicuidad del RNAPII en un residuo específico de lisina (RPB1 K1268).
Investigó el impacto de la ubicuidad de K1268 en la reparación del ADN y la degradación del RNAPII.
Se examinó el papel de la estabilidad del RNAPII en la recuperación de la transcripción y la respuesta celular al estrés genotóxico.

Principales resultados:

La ubicuidad de RNAPII en RPB1 K1268 actúa como un punto focal para coordinar las respuestas al daño del ADN.
La ubicuidad de K1268 es esencial para la reparación del ADN y señala la degradación del RNAPII, promoviendo la supervivencia celular.
La degradación del RNAPII conduce al cierre de la transcripción, y su agotamiento persistente dificulta la recuperación de la transcripción.

Conclusiones:

La regulación de los niveles globales de RNAPII es parte integral de la respuesta al daño del ADN celular.
El tamaño del grupo de RNAPII puede influir en los programas de transcripción específicos de la célula en trastornos de inestabilidad del genoma y células normales.

Palabras clave:

Daño en el ADN La ARN polimerasa II La irradiación UV transcripción Ubiquitina y sus derivados ubicuidad

Más Videos Relacionados

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

Published on: March 20, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Videos de Conceptos Relacionados

DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Nucleotide Excision Repair

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

Nucleotide Excision Repair

Nucleotide Excision Repair

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.