Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Viral Structure00:56

Viral Structure

73.5K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
73.5K
The Replisome03:01

The Replisome

37.8K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
37.8K
The Replisome03:01

The Replisome

9.4K
9.4K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

607
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
607
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

558
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
558
Leaky Scanning02:28

Leaky Scanning

5.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Structure and receptor recognition of type-II feline infectious peritonitis virus spike glycoprotein.

The EMBO journal·2026
Same author

Genomic and structural evidence of SARS-CoV-2 and MERS-CoV in migratory birds.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Hemagglutinin double-mutation enhances binding of human-infecting avian influenza virus clade 2.3.4.4b H5Ny to human and SLe<sup>X</sup> receptors.

EMBO reports·2026
Same author

Localizing the Glycan Binding Sites of SARS-CoV-2 Receptor-Binding Domain.

Analytical chemistry·2026
Same author

Disease-stage-specific immunometabolic remodeling in pediatric obstructive sleep apnea: a single-cell transcriptomic atlas of adenoid tissue.

EMBO molecular medicine·2026
Same author

Comparative Transcriptomics and Metabolomics Analysis Revealed the Mechanism of Exogenous Salicylic Acid Improving the Cold Tolerance of Walnut.

International journal of molecular sciences·2026

Video Experimental Relacionado

Updated: Dec 25, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

4.1K

Conocimiento estructural de la maquinaria de replicación de arenavirus

Ruchao Peng1,2, Xin Xu2, Jiamei Jing1,2

  • 1CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Nature
|March 28, 2020
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores han determinado las estructuras casi atómicas de las polimerasas de virus Lassa y Machupo. Estos hallazgos revelan características únicas y un estado inherentemente activo, crucial para comprender la replicación viral y desarrollar nuevas terapias antivirales.

Más Videos Relacionados

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

9.1K
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

10.0K

Videos de Experimentos Relacionados

Last Updated: Dec 25, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

4.1K
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

9.1K
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
08:34

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

10.0K

Área de la Ciencia:

  • Virología
  • Biología estructural
  • Biología molecular

Sus antecedentes:

  • Los arenavirus, incluidos los virus Lassa y Machupo, causan fiebre hemorrágica severa y enfermedades neurológicas.
  • Las polimerasas de ARN dependientes del ARN viral son esenciales para la transcripción y la replicación viral y representan objetivos antivirales clave.
  • La base estructural de la función de la polimerasa del arenavirus era desconocida antes de este estudio.

Objetivo del estudio:

  • Determinar las estructuras de resolución casi atómica de las polimerasas de virus Lassa y Machupo.
  • Esclarecer las características estructurales y los mecanismos reguladores de las polimerasas de arenavirus.
  • Proporcionar una base estructural para el desarrollo de nuevas terapias antivirales.

Principales métodos:

  • Se empleó la cristalografía de rayos X para obtener estructuras de resolución casi atómica.
  • Las estructuras se determinaron tanto para las formas apo (no unidas) como para las ligadas al promotor de las polimerasas.
  • Se realizó un análisis estructural comparativo con otras polimerasas virales.

Principales resultados:

  • Las estructuras revelaron una arquitectura general conservada con características locales únicas, incluido un dominio de inserción específico de arenavirus.
  • El sitio activo de la polimerasa del arenavirus está inherentemente activado, a diferencia de las polimerasas de influenza y bunyavirus que requieren activación alosterica.
  • La dimerización puede facilitar la actividad de la polimerasa.

Conclusiones:

  • Las estructuras determinadas proporcionan información sin precedentes sobre el mecanismo de la polimerasa del arenavirus.
  • Las características estructurales únicas, en particular la polimerasa inherentemente activa, ofrecen nuevas vías para el diseño de fármacos antivirales.
  • Estos hallazgos son críticos para avanzar en la comprensión de la replicación del arenavirus y combatir las amenazas a la salud pública.