Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

3.3K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.3K
Colloidal precipitates01:09

Colloidal precipitates

4.5K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
4.5K
Other Unique Bacteria01:18

Other Unique Bacteria

322
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic...
322
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

62.8K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
62.8K
Bacterial Signaling01:30

Bacterial Signaling

39.8K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
39.8K
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

4.0K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
4.0K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Hot exciton dissociation in graphene nanoribbons.

Nature communications·2026
Same author

Chemical and physical equilibria shape dual ice-nucleation pathways in an organic crystal.

Communications chemistry·2026
Same author

Cyclophane-based shielding strategy for singly dispersed graphene nanoribbons.

Nature chemistry·2026
Same author

Rethinking energy transition strategies for the European Union amid rising energy prices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Design Principles for β-Solenoid Stability via Covalent and Electrostatic Capping Motifs.

The journal of physical chemistry letters·2026
Same author

Multiphase Chemistry and Phase State Explain Nonlinear Effects in the Formation and Evaporation of SOA from Mixed Monoterpene Precursors.

ACS ES&T air·2026
Same journal

Radical Cascades on Seawater Microdroplets Drive Atmospheric Mercury Oxidation.

Journal of the American Chemical Society·2026
Same journal

Superior Selective and Fast NH<sub>3</sub> Adsorption of Soft Porous MOF/Ionic Liquid Composites with Ordering Phase Transitions.

Journal of the American Chemical Society·2026
Same journal

Systematic Catalyst Variation for Improved Stereoselective Epoxide Polymerization: Subtle Modifications Resulting in Superior Efficiency.

Journal of the American Chemical Society·2026
Same journal

Deciphering the Halide Chemistry of Cl<sup>-</sup> and Br<sup>-</sup> in Enhancing Kinetics of Mg Plating/Stripping.

Journal of the American Chemical Society·2026
Same journal

Electrosynthesis of C<sub>6</sub> Chemicals by Propylene Oxidative Coupling on Au Surface.

Journal of the American Chemical Society·2026
Same journal

Statistical AI Enables Precise Screening of Multielement Catalysts.

Journal of the American Chemical Society·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Dec 25, 2025

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
08:46

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Published on: January 15, 2014

9.5K

Las interacciones electrostáticas controlan la funcionalidad de los nucleadores de hielo bacteriano

M Lukas1, R Schwidetzky1, A T Kunert2

  • 1Max Planck Institute for Polymer Research, 55128 Mainz, Germany.

Journal of the American Chemical Society
|April 1, 2020
PubMed
Resumen
Este resumen es generado por máquina.

Las proteínas nucleantes de hielo bacterianas (INP) se agregan a través de interacciones electrostáticas, mejorando su eficiencia de nucleación de hielo. Esta agregación es dependiente del pH, crucial para la formación de hielo bacteriano bajo estrés.

Más Videos Relacionados

Electroporation of Functional Bacterial Effectors into Mammalian Cells
08:39

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Published on: January 19, 2015

10.4K
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

11.1K

Videos de Experimentos Relacionados

Last Updated: Dec 25, 2025

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
08:46

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Published on: January 15, 2014

9.5K
Electroporation of Functional Bacterial Effectors into Mammalian Cells
08:39

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Published on: January 19, 2015

10.4K
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

11.1K

Área de la Ciencia:

  • Microbiología
  • La biofísica
  • Ciencias de los materiales

Sus antecedentes:

  • Las proteínas nucleantes de hielo bacterianas (INP) son altamente eficientes para promover la formación de hielo.
  • Su alta actividad está relacionada con la formación de agregados funcionales de INP.
  • El mecanismo exacto de la actividad de INP sigue siendo incompleto.

Objetivo del estudio:

  • Investigar el papel de las interacciones electrostáticas en la actividad de nucleación de hielo de los INP de *Pseudomonas syringae*.
  • Para correlacionar el estado de carga de los INP con su eficiencia de nucleación de hielo.
  • Para aclarar el mecanismo detrás de la formación de agregados INP activos.

Principales métodos:

  • Utilizó un ensayo de nucleación de hielo de alto rendimiento.
  • Espectroscopia de generación de frecuencia de suma específica de la superficie empleada.
  • Se determinó el estado de carga de P. syringae no viable en un rango de valores de pH.

Principales resultados:

  • La actividad de nucleación de hielo de los agregados INP está fuertemente correlacionada con la carga neta, mostrando una actividad mínima en el punto isoeléctrico.
  • La actividad del monómero INP es menos sensible a los cambios de pH en comparación con los agregados.
  • La alineación interfacial de las moléculas de agua, indicativa del estado de carga, se relaciona directamente con la actividad de nucleación.

Conclusiones:

  • Las interacciones electrostáticas son esenciales para la formación de agregados INP altamente activos y funcionalmente alineados.
  • Este mecanismo explica cómo las bacterias promueven la nucleación de hielo bajo condiciones de estrés.
  • Los hallazgos proporcionan información sobre la base molecular de la nucleación del hielo bacteriano.