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¿Qué se esconde tras la hidrólisis de la base de Schiff? Química dinámica covalente para la captura precisa de los
Yuting Xiong1,2, Xiuling Li1, Minmin Li1,2
1CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, P. R. China.
Journal of the American Chemical Society
|April 4, 2020
Resumen
Un nuevo método de hidrólisis de base de Schiff captura con precisión los glicanos sializados (SG), cruciales para el descubrimiento de biomarcadores de cáncer. Este enfoque de química covalente dinámica mejora la glicoproteómica y la detección del ácido siálico.
Área de la Ciencia:
- La bioquímica
- Química analítica
- Ciencias de los materiales
Sus antecedentes:
- La expresión aberrante de los glicanos sializados (SG) está relacionada con la progresión del cáncer y la metástasis.
- Las glicoproteínas sializadas son biomarcadores de cáncer establecidos.
- Los métodos actuales de identificación y análisis de SG son complejos y requieren una mejor preparación de muestras preanalíticas.
Objetivo del estudio:
- Desarrollar un método innovador y eficaz para la captura de SG de muestras biológicas antes del análisis por espectrometría de masas (MS).
- Para utilizar la química covalente dinámica para el enriquecimiento SG preciso.
Principales métodos:
- Se empleó una nueva estrategia de hidrólisis de base de Schiff.
- Se sintetizó y caracterizó el gel de sílice modificado por la base de glucopiranósido-Schiff.
- Se evaluó el rendimiento del material en la captura de glicopéptidos sializados en cuanto a selectividad, capacidad y recuperación.
Principales resultados:
- El material desarrollado demostró una extraordinaria selectividad de enriquecimiento (relación 1: 5000 con interferencia).
- Se logró una alta capacidad de adsorción (120 mg·g−1) y recuperación de enriquecimiento (95,5%) para los glicopéptidos sializados.
- El material base de Schiff exhibió una fácil hidrólisis, una característica única para los materiales de enriquecimiento.
Conclusiones:
- La estrategia de hidrólisis de base de Schiff ofrece un enfoque altamente específico, eficiente, suave y reversible para la captura de SG.
- Este método avanza significativamente en la glucoproteómica y la detección del ácido siálico.
- El enfoque es muy prometedor para el descubrimiento de biomarcadores de cáncer.
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