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Una vía de translocación para la secreción de proteínas no convencionales mediadas por vesículas

Min Zhang¹, Lei Liu¹, Xubo Lin²

¹State Key Laboratory of Membrane Biology, Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.

|April 10, 2020

Resumen

Los investigadores descubrieron que TMED10 actúa como un canal de proteínas, facilitando la secreción de proteínas sin líder a través del transporte de las vesículas. Esta vía regula la entrada de carga en las vesículas, crucial para la secreción de proteínas no convencionales.

Área de la Ciencia:

Biología celular
Biología molecular
Secretión de proteínas

Sus antecedentes:

Las proteínas sin líder se secretan a través de vías no convencionales, a menudo involucrando vesículas.
El mecanismo de entrada de carga sin líder en las vesículas sigue siendo poco conocido.
El tráfico de vesículas es un proceso clave en la secreción celular.

Objetivo del estudio:

Para aclarar el mecanismo por el cual las cargas sin líder entran en las vesículas para la secreción.
Para identificar a los actores moleculares involucrados en la regulación de la entrada de la vesícula de carga sin líder.
Comprender el papel de los canales de proteínas en la secreción de proteínas no convencionales.

Principales métodos:

Ensayos de reconstitución in vitro con liposomas.
Estudios de localización celular de TMED10.
Análisis de las interacciones proteína-carga y la oligomerización.
Investigando el papel de HSP90 en el traslado de carga.

Principales resultados:

TMED10 identificado como un canal de proteína que media la entrada de la vesícula de carga sin líder.
TMED10 La región terminal C interactúa con un motivo de carga para su liberación selectiva.
TMED10 transloca directamente las cargas sin líder en liposomas in vitro.
La translocación es dependiente del despliegue de proteínas y mejorada por HSP90.
TMED10 se localiza en el compartimento intermedio ER-Golgi, dirigiendo la entrada de la carga.
La unión de carga induce la homooligomerización de TMED10, formando potencialmente un canal de translocación.

Conclusiones:

TMED10 es un componente crítico de la vía de translocación para la secreción de carga sin líder.
La interacción con la carga TMED10 dicta la liberación selectiva de proteínas.
TMED10 funciona como un canal, posiblemente como homooligómeros, para la entrada de las vesículas.
Este descubrimiento arroja luz sobre un mecanismo fundamental de la secreción de proteínas no convencionales.

Palabras clave:

Compartimiento intermedio ER-Golgi La galectina HSP90 y sus derivados HSPB5 y sus derivados TMED10 (en inglés)inflamación Interleucina 1 y sus derivados Tráfico de membranas Translocación secreción de proteínas no convencionales

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